TBX-35

WormBase ID: WBGene00006554

WormBase description: Is an ortholog of human EOMES (eomesodermin) and TBR1 (T-box brain transcription factor 1). Exhibits sequence-specific DNA binding activity. Is involved in cell fate specification; pharyngeal muscle development; and positive regulation of transcription by RNA polymerase II. Is expressed in several structures, including MSaa; MSpa; MSpp; head neurons; and intestine.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: TGAAGGACTGGGAGTATTCA

sgRNA2: TTCAATGCTCTCTTTTGATA TBX-35-NKIsg3: AAAGAGAGCATTGAAGGACT

5'HR: GCATGCATCAAAAAATGGTACGATAAAAATTTCTTCGTTGGGGAATACTTTTTCGATTGAAAATAATTACCTACCGTATTAAAGACGGACTCAAAAAGTTTTTGGTAAGCTTCAAAAAATTGTTTAAAAAAAAAACAAAATTGCAGAGAAAAACTAAAAACTCTCTAATTCTTATCCATACTGCGCAGTCCCAAAAAATTTATTTTTGTGGGCAAAATTTTTGAAAAGTTGGCAAGCATTGGGTAAGAAGTTTGAGGACCTCGGTCAGTGGCTGCAAATAGGATCTAGACATTGCCTCAACATCCATACAAGTTTCCAATCAATATCTACAACTATGTGGTGTGCTTCGAACTGGATAAGTATAGCGTATACCCGAGAGCATAAGTATGTATACTTGGTGCAATTAACCAACAGTGTACGGTTGTATGCTCGTACTTTAGTTTTTCGAAGTGAGGAGCTTGCCAACTTCAGAATCTCTACTTGTTTGTGCTGAGTACTTTTTTGTGATCCTAGTAGGTATACGAATATGTCAAGAGAAATCGGAAAAATTCAAGATTCTTGATCTGCAATTAAAGATTAACATGTTTCAAAATATAACCAGGATTAAGTTTTCAGGCGAATAGAACATCTGTAATTCCAGGCATACTCCGATTAGCACATTGTGACAGAGTAACAACAAAATCTGAACATTGCCTCAACATCCAAGTCTCCAATCAATATCTAGAACTCCGTGGTGTGCTCCAAACCCGATAAGTCTGGCGTATACCTGAGCATGAGTATACTTGGAGCAATTAACCAAAAGTATACAACTGTATACTCGTGCTTTAAATTTTCGAAGTGAAGTGAACAGTATACTACCGTAGTTCACGCCCAAAAACGCACACGCTCATTCTGGATTGAATCCGCCTATTTTTTGAAGATCCCAAAATTATTTCAGAATCAGTCATTCATTGTTTCTGGTAATCTCTGAAACGGTACC

3'HR: CTGCAGATGTCGTTCAACTATTCTATGAATACTCCCAGTCCTTCGATGCTTTCCTTCGATATGGATATCCCTATCACAGCATTTCTTCATAATTCTGATGTCTTTCAATTATTTCATCCAGATATTATGGAACAGGTTGTTGTGAATAAGGAACGAGTCTTTTTTCCAACACCAAGTTTTAATGTTTTGAATTTGGAGTCTGATTGTCTGTACAAAATGAGTCTTCGATTTGATAAAACTAATAACAAACGGTTAGTTTTATTACGAATAATTGAAGTTTGAAATAATTTAATCTTCAGATTCCAATTCAACAGACAAACCAAACAGTGGACTGAATACGATAAAGTTTCAAATCATAAAGAAAGTGAGGTTTTCGTTCACCCAAGTGAAATTCGAACAGGAGATGATTGGATGAAATCTCCAGTTGAATTCTTCATCAAAGTGACAAATCTTAGAAGTTCGGAATCTGATGAAGTGGTTAGTTGTTATAAAATAGTGGAAACAATTTTTATTTTTTATTTCGAAAACCACCCACTAATTAGGAAGCCTGAAATTCAGAATGTAATTTTTTTTCCAGATTTGCCTCGAAAGTCAGTGTAAATACCAGCCAACACTTCTGATCCAAAAAGTCTTCAGAGATTGTTTTGGGTGTTTTCAAGCACACGGTCCTCCAGTTGAAATAAAGTTTGCCCTTTTAACTTTCATTGCAACAACACGATATTGTGTAAGTTTCTAAGTTTTTGCTCGCATTGAATTTTCAAAGATCATATTGTTTTAGAGCAGAAGAATTGCGGATATAAAGAGCTCACTCAACCCATTTTCCAGTCAAGACACCAAAAAGAAGAATAAGAGGAATACATTGAAAGCTGTATCGCAGGAAGAAAATCAACCAATTGAGAATAGCAGCTTTCATCAGAGTTTTTCGAATCAATATCTGTCTAAACCTGATCTTGACCTAAAAAGCACACTAGT

KI verification primers: TBX-35-NKIF: 5'-AACGATCTAAGAAGGCGACACG -3' TBX-35-NKIR: 5'-AAGGATTGCTGACAGAAAATAGAGTC-3'

Download

Strain name: SYS686

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; tbx-35 (devKi223 [mNeonGreen::tbx-35]) II

Expression pattern: Not expressed

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA