KI information : 	mNeonGreen knockin at N-terminus
sgRNA1 : 	CAATCGAGTCGGACAATCTA
sgRNA2 : 	GACTTTGTCGCTGCTGCCCA
KI-homologous recombination fragment-5'arm : 	GCATGCACCAAGTTTCTGGAGTAGGGATTTTAAAACTGCCAGGGTTCGTTTGGAATTACTGTAAATTTCCTATCCTGCTATCGTTTTTTCAGCTAAACTTTTTTTCTTGAAGATATAAGTTAACCAACTGAAACATAGAGCATTTTGATGGGAAATTTGTGATTGCAAAACTTTAGAATTTATTTTTGAAAAGTCAGCATCTACTTGGAAGAACAACAATATAACTGACCTATCTCAAATCACTGTGTTACTTCAACTGCATTTCCTATAAAATTTAAATGATAGTGAGATATAATACAACGTAGATACTCCCTAAAAGGCCATTCCATAATGCCTTTTTTCTTTTTGCAACCATTATTTTTTCTTTCATCGGTCCCTCTTCCCTGTCTAATCCTTATTGTCTTACACACTCTCTCGGATGTTCTCTCTGTCTCTTCTTATAATCAAGTTTCGATCGATATTCACTAGCTGTTGTGAGGTTAGCGTTTTAGAAGCTGCACCAGAAAACCGAAAAGAGTGTTGGAGACGAAGATACGCAGATGGTCCGCCTATGAAAATTCTTGATTCATAGCGTCTCCTACCTTGGAATGAATTTATATTGTTAATCTATCCGCTTTACCTTTTTCTGGAACATTTAACATAAATGTTCGTTTATAGGAGAATATCACAAATCTCTCTTCACTCAAACCTCCACTTTCTGACTGTACGAGTCAAGATCACCGAATCAAGATCACCAATGTACTTTCCAGAAATTAAGCGTTTTGTATCGATTCTACATAAAATGAGACTTACCATTTTTAATTAATTAGTTTTTTCTCATATATTCGCTTGTTCTCTTTATTTTTCAAAAAAAAAAATTGAATTTTAATATAAATTTAGAAATCTAAACACAAAAAATTTCAGGGTAAGGTACC 
KI-homologous recombination fragment-3'arm : 	CTGCAGATGTCGTCGGAACCAACTTCTTCAATCGAGTCGGACAACCTACGACGCTCGAAACGAAAGAAGTTCAAGCTCGACTTCGTTGCCGCCGCTCATGGCAACAACCAGAAAAAGAGTCGTAAGGATCCTCATGGGCAGGGAGATGATGATGACGACACTTTTGATGACGATCTGAATCATTTTGAGCCATTGAGCGTTCTCACGGAGCAAGACGTTTCGATGGAAATGTTTGAAGATGATGAAGAGGATACTGTAAGCCGACGGAGGACGAGACGAAGCACGGCTCATTTTCAGGATTATCAAGAGCCTGACAGATGGATTGAGGTTAGAATCTTAAATTTCAATTTTTTTCAAACAATTTTTGCAGAACTCTGGCCCACATGCTTGCCATAAATGTCCATCCCGGTACGAGTCGAAAAGCAGTCTGGCGAACCACACAAAAATGCATCTTGGCGAGAAGCGGAAGTTTGCCTGTGAGCTGTGTGATTTCTCGGCGAGCACACTGAAATCTCTCACTCATCATAACAATATTCATCAGAATTTTGGGGTTCTTAGTCAGCAGACGAGTCCCGTGATTCCCGTGGCAAGCTCGGCAGATTCTACACTCAATTCTTCTATAAACTCCACTGGATGCCAAATCAATGCTCCACCTGCTCCGAAGCTCGACGAGGAAGCTCCAATTGCCGTAGAATCGGTAGTAGTTCATCATGAAGACGTTGCCGAATTTGACACTACACCTCCACCTATACTAGAACGTGAGGATGATGGACCTCCGGTATTGATAAGAGAAGCACCGGTTCGTAAATCGAATCGTCCAATGAAACCAACTCAAAAAGCTCAAAAAATGACAAAGCAACGAGAGCGGAAGTCGAAAACAGTTGAATCACCGAAAGGATCACTTCCATCATCATCCGCATCTTCAGTAACTCCACCACCAGTGAGAAAAGATGTCGAAAAAACTAGT 
KI verification primers : 	SPR-4-NKIF: 5'-TTTACCACAATTTTTCAACTGGGTT-3' SPR-4-NKIR: 5'-GTAGTGAAAGGACAATGAGGGCA-3'