KI information : 	mNeonGreen knockin at N-terminus
sgRNA1 : 	TTCCGTTCAGAAGCTGATTT
sgRNA2 : 	ATTGAAGACTGAATAACTTT
KI-homologous recombination fragment-5'arm : 	GCATGCAAGCTGTTCTAGAAATGTTTTAAAAAACAATATCCAAGTTTAAAAAAATCTCAATTTATTTTTAATTTACGAGGTTTCCATCTCAGCGTGTGACACTTTTCTTATAACTTGGCACCTACATGTATCCGGTGTGAATTTTTTCTGTTGCTGTGTGAGCATTTCCAGCAAACCGGAGTTCTCAAAATCAGTAAGGTTATATGTTAAAATTAAACTGGCTTATTATTTTCAGAAAATGAAAAACTGCCATATTTGAGTGATAAACTGAATGAATCCGTAAAATTTGGTAGTTGTATGTTCTATGCATTATCATATACATCAATCTGGTAATTTTTATTAGAATCAGATCTTGAATATTGGAAACTTTGCGAATTAAAGACAAATAATGATTAGGCAAGCTGATTAAATGAGTGATAAAAATGGTTAATGTCAATTCACAGTCTCCGCCGAAATTTCCCAGGCTGTACCCAATCAATATCCAGTCGTGGCAAAACTGGAGATGTCCTGACTCGTTCTCATGAGAACCTCACTGATGAACCATGGGAACCCGTGATGGGGACTGTAAAAATAGAGATTTCAGAATAATTCCCATGGCTCCAAACGCTTGATACAAAGCTCCACCCTCTGAACAATAATGGAACTATAAAAGGTAACCATTTATTCATTGTTCATTTACTTTCTTAGTGGATCACTGGTCTGCTGGTTTAGTATACTTTTTGATAATTGTAACATCAGGAAAGTGTACTGATTTTGATCAATTACTTTTAAAGCATTCAGTGAACTGTATACTTCTAGAAAAACAGTACGAGTTATCTCACTAAACTAAATCAATTTGTGCTGCTTTCTTGGTCCCATTTATCTGTTTTGTCACAATTATTCCTTTTTAACGGCTCATCTTGAATTTTTATAAATTCTACATTTCTTATTGATTTTAAATTAATGAAAATTATTAAATTTCAGGGTACC 
KI-homologous recombination fragment-3'arm : 	CTGCAGATGCCAAAGGTCATCCAATCCTCAATGAGCGATTATCGTTCCGTCCCATACAATCAAACTCCAAAGTCGGCCTCCGAACGGAAAAGAAGAAATATTACAAACGAGTTGATTAACGAATGCAAGACGATTGTTCAAAAATCAGAAGAAGAACATATATCGTAAGTTTAAATACTCTGTTAGCTTAAAATAGTTATTAAACTAATTCATTTTTTAGACAAGAAGTAGTTCTCTTCCGAATTGTGAAACTTGTGACTGGAGTTAATCTGGAATCTAATTTCTCATCGAATGACCTCTCCGAATCTACAAGACGGAAGTTTGATACTGAATCCGAAAGAAGAAAAGTGAAGACTGAACGTGAAAAGATTCGAAGAAAGAAACAAGATGACTGTTATGCTGAATTGAAATTCTTCATTTTGAACAAACAAATGGGAAGTTACGAGCAACGGTAAGAGTTTCATTATGGCTAATAAAAATTCAAAAAATTATTTTCAGATTAAAACTTGAACGGATCACAATTCTAGAAATCATCATTGATTACATTAAACACAATTCCGATCTTCTGTATCCAGAAACAATTCCCCAAATACTTCCTCTTTTAGCTGGAAAATCAACTGCTACTTGTGAGAACAAAGAGAATGAAAAGCCAAAAACACGAATGGAAGTGAAGGATCTCTTCCCAAGATTGACGTTTCAAGAAGTTCAAGAATCCCCAACATCTACTTCTCCATTGCTCACATTTCCATGTATTCCAATGATCCCAACTACTCAGTTCAATGTCTTGTCAAACTACAACACCGTTCCATCAATATTTTCTGCTCCCCTCCGATTCATTCTTCCATCACTTCAAATCTTAACACCAGAAACCAGTGACGAGGAAGAAAATGAAGAAACCCTCGATATAATTAATTAGTCTATCGATCTCCTGCAAATTTACAATTTCTGAAAAAAAACTTATTACTAGT 
KI verification primers : 	HLH-25-NKIF: 5'-TTGGTAAAATCGGAGCTCGATG-3' HLH-25-NKIR: 5'-AAACCAGGAAAGTTTTGACAGCC-3'