KI information : 	mNeonGreen knockin at N-terminus
sgRNA1 : 	GTACTGATGAGGACCATTTC
sgRNA2 : 	TCATAACAGAAAAACATCAC
KI-homologous recombination fragment-5'arm : 	GCATGCATCTTCTTCTCATTTTTTTGTTTGTTTTTTAGTTCCAGTATTTACTTTAGCTGTTTTGAGATTTTGAGAAGTGTATTTAGTATACATTCCTAATTATTTTTTGCCCTAAATTCTTTAGAGTCTTACAGTAGCAAATCTACAGTAGTCTATAGCTATGGTTAAGAGTTTCAGCTACAGTAGCAACATAGTTCTACCTCACCAGAAATAGTATTGCTACTGTAGCGCTTCCAATTTCTAGTTTTCCTTAAATTAACCAATTCTCACTTCATTTTAACATTCTTCCCCCGAATTCTCTATGTCTCACCTCACTATCAGTCTTCCAAATTTTCACCTCAAGTACAGTACATCTGAAACTCTAACACCTCCCCATGCATTTTACTGCCTGTAGATCCCTCTTGTTTACCAACTTCTTTCATGTTCTTCCGGTCTGCCGTTTCACTTCCTTTTCTCACCTTGTCGGACCCCTAGCTCTCCATTAAAGGATTAGTACCGCCTGAGCATCCGCCCGGCTCTTCACAGTGTCGCCTCTCACCAGGGGTTATCAAACCAATATGGTCAAGAGTTGTGGGAAACTGTGTCATGACTCAAGTGCTTTTTTCCCATGCACACTTATCAAATGATTCCCACACTGATAACTCGGCACGTCTTTTGTGGTTGATAGTGATGGGAACCATTGGGATATAATTTTTTTTGGCGAAACGACACAAAAAGTGGGTTTTTAGGGTTTAAGGCAATTAAATTTCTACGAATAAGATTCTAATCTAGAAAAAGAAGGCGTGCTGTAGAAGTGAGGGGTACTGTAAATCGCCACGCTCCCTCTTCCGAATGGTTTATGACTTTTCTCTATATCTCCTTCTCTTTCTCTATATTTCTCAAATTCCGCAACATTTCTCCCTTCTTGATTTTGTTCATTTACAAATCGAGCTATAAAAATCAATAAAAACTTAATTTTTTTTCCAGAAGGTACC 
KI-homologous recombination fragment-3'arm : 	CTGCAGATGGTCCTCATCAGTACCCCACCACCAGCTTACGCTCATAACAGAAAAACTTCACAAGAGAAGAAACGACGAGATGAGATTAATGCAAAGATCAAGGAGCTTCAACTATTGATTCAAAATGAATCGGATAATGAGAAAATGACGTGAGTTTTCTATTCGTTTAGTGTTATCTTCATTCAAATCTTACTAATTTTCAGCCAAGGTGATGTCTTAAACCGTGCAGTCGAAGTGGTCAGTCGCATGGAAACCGAATCACCTGGACCATCATCAAATCCAAATCGCAAAGGATTCTTTGACGGATTCCGATCAATCGAGTCGCTCACTTATTCCTTCATCAAATCCCTGGGCGTCAATTCAGATGTCTGTCAGGATTTTGTGCAACGTGCCAAGCAGTTTTTCGATCGAGAACGTTCATCTTTGCTGAGCACTGTATCTGGAAAATCAAAGAGAAGATCTGAGTCGGAAATTCTCCACTCTTCAATGAGCTATAGATCCCAGTCAAGCTCTCCATCGACATCGGAATCTGGAATCACAATTGATAGAAAGGAAGTGAAAAAGAATAGAGAGCAAGATAGAAGAGATCGTCAGGGAGAAGCTTTTGATGCTTTGAAGAACTTTATCATTGAGAACAAGCTCATGACATCTCATCAAGTGTAAGCTTAAATTTTCCCAATACCATAAATTAAACTTTTCCTTTTTTCAGCGAAAAAATGCAACGCTTGAACACCCTGGATATCATAATTGCCTACATTCAAAACAAGAAGCACAATTTTGTTAGTCGAAGCGACCAGGAGCAGTCACTCTATGCACATGCAATTGCAGAGGGAAAGAAGACTGCAAAAAACATTGCCTTCCAATTTTTCAAGGTGAGTTGTCAGGAACTTCGAGAAGATTTTTTTAAATGTTATCAATATATTTTCAGAGCGACCGTCACCTCGTTGTCCGATGCGCAGACCTTACTAGT 
KI verification primers : 	REF-1-NKIF: 5'-ATTAGTCAACAAGTGGGGGCTATC-3' REF-1-NKIR: 5'-AGTGAATATGATGGAGACTGTTGAGAT-3'