KI information : mNeonGreen knockin at N-terminus sgRNA1 : TGAGATGGTGAGCCGGGAGG sgRNA2 : TTTACAGATGACGTCGTCGA KI-homologous recombination fragment-5'arm : GCATGCCCCACCTTAACAACTAATTCATTTTCCAATTTCCAGGTTCGCCGACCAATAGAAATGCGCAAAAACCACATCCAACAACGCTACCGCCGAAAGAACAAGGAAAAAGAGTCGTCGGCGGCAACACAGATCTTCAACCAACTCCTCACACAAATGCCGACGATGGCGACCGGCGGAGTGAGCACTGATGGAGCCATCAACACATTCAATTTACTCGAGCAAATCTCGCAATTCACGCAGGCTCAAGAGCTCATGAATAGCAGTGCCACATTTTAATTATCATTATTATTTTTTCCCCCTTCACATCCTTTTTATGCACAATACCTTTATGTAATTTTTCTTATTTATTGAAATTTGAAATTTGAAATTCTCCATAATGTATTATTGATTAATAAGCAATCCTTCATCATTCCATTACCCCTTCAGAAAAATACAGTATTAAATAATTTTCCGCCAATTTTCTAATAATAATTGTGCCCCATTTCCCCCAACTTCACCGCGTGTTTCCGACTGTGAGCTTTACCTCCTCCTCCGACAACTTATAATAATAGTTAGGCTCCGCCCCATTCTGCAGGAGGCCCCTCCCCCAATTCTCTGTGCATGGTTGTGTTATATTCACCTCCTCCCACCACTTTGTCATTTCACTTGAATCATCATTTTTGCATTTTCATATTTATTTTGAAAAAATCTCATCTCACTCTCTCTCTCTCTCCTTATCTCTTGTGGTTTTTGACGGATAAACGTATGTATCTCATTCTATCACTTTTAGCTTTTAACTTCGTCCTCTCGCTCTCTCGCACTCTCCCCTCGCTCCACAGTCTCTCTCCGTCTCTCTCTCTCTCTCGCTCTCCACTTCTTCTTCTTCTAAAACAACGTCACACGTGATTTACAGGGTACC KI-homologous recombination fragment-3'arm : CTGCAGATGACGTCGTCGAAGGAGGAGATGCCGGATGAGATGGTGAGCCGAGAGGTTGAGGAGGAGATGGAGGATCGGGTTGAGGAGACGGCGACTACAGCAGCAGCGTGCACCGGATGCGCTCAGCTTCACAAGGATATTCGACAGGTGCATATTATTTTGTTTAGCAGTAGGCTCACGCGCCTGCCTACTCTCGACATTTGGCTCCAGCTTCTTGCCCAAGTTTGGCCTAAAAAATATTAACTTGGATAAGAAGCTGGCTAAACTTGGGCAAAAGTTGGGCAAGACTTTGGCAAAACTAAAATCTGGTCAGACTTCTACCCAAGTATAACTAAAAAAAAGAAAATTTTGGACCAAAGTTTGGTAAGAGTTGGGGGAAACTTTGGTGCAGCCTGAATCCAAGTTTTGCCCAAGTTTTGCCCAATTGCCAACTAGAGCCAAATGCCGATAGGCAGGCATACATGAAGTAATTCTCGGATTACCAGAAACATTAAATTATTTTTCATTTCATTGAGAATTTCTGAAATTCTAGGAATTTGAATCGCCGCGCTGAAAGCTCCGGTCTATTCGTTTTCTTACAGGATGTTTCAAAAATGATGTCGAAAATTGACGAAGTGTGCGGAAGGCTTGAAGCTCTTATCAGTGAAAAGGAAAAAGTCCTGCTTGAGCAAATGTCCGAGAGCGGAAGCGAGGAGAAGGTGAGATTTTTGACAACTTTTAGCGGCCATGTTGCAAAAGTTCTGCGGGAAGGAGCTGGTGGAACTTTAGAAATAAGCCAAAATGGGCTGAAACTGAGCTTATTTCGCAGCATTTTCCCGCCGCCTGACAATTTTCGAGAAAGAAACTGAATCTGTTTTAAAGTTATTTTCTCAGTTTTTCGCGATTCTGAGCCATTCCCAAGAAACTTGTTTATTTTTGCATCCACTAGT KI verification primers : ELT-6-NKIF: 5'-TTAGAAGCTCCTAAATGCCTAAGGA-3' ELT-6-NKIR: 5'-GTCACATTACTTGAAGAAACAAAGAAA-3'