KI information : mNeonGreen knockin at C-terminus sgRNA1 : CTGTACTGAAAGTCGTACGC sgRNA2 : TCCGTCCAAAGTGTAGGTGT KI-homologous recombination fragment-5'arm : GCATGCAAAACATCATTTTCTATACAAACATGAGTTTTTATTTTGATGAATTTGAATTCAACACAAAATTTGAATTTTTTTTGACACCCATATTAACTATTTTCGGAATATTTTCAATTTTAGGCCATTTTAGGCCTGAAATCGGCCTGAAACGGCCTAAAAACCTCGAATTTTGGAACATATAAAAAATACGTGCACAACTGGTAGGAGTTTTGAGTTTCATGACCAAAAGTGTAAAAAGAATAGAAATTTCACATTTCAGGCCCGAAATCGGCCTGAAACGGCCTGAAATTTAATATTTCAAAACATAGTCAATATGAGTGTCAAAATTTTGCAAATTTTGTGTAGAATCTGAATTTTTCAATTTGGCAAGCCTTATGTGTCCCAAAAGTGTCCCAAATGGTGTCCCAAACGACACTCCGAAATTCCGCTCGGCTATTTTTCAGTGAAAACATCATTTTCTATACAAACATGAGTTATTATTTTGATGAGTTTGAATTCAACACAAAATTTGAATTTTTTGACACCCATATTAACTATTTTTCGAAAAATTTTCAGTTTTAGGCCTGAAATCGGCTTGAAACGGCCTGAAAATCTCGAGTTTTGGAAATCAACCAAAATACCTGTACAGTTTGTGAAAATTTAACAAGTAATTCAAGTTTATTGATCAAAAATGTTGAGTATCAAAAATTTCACGTTATAGGCCCGAAATCGGCTTGAATCGGCCTGAAATTTAAATATTTCAAAACCCAGTCAATATGGGTGTCAAAATTTTGCAGATTTTGTGTAGATCCCGAATTTTTTAGTCTTAAAAGTCCTAGATATCCCCAAATGTCCCAAACTTCAATTTTCAATCTTAAAAATCCTAATTTTCAGCCAGCTACCCTGCATATGATTTCCAGTACAGTCAATCCAACACAAATTCCACTAACACTTACACTTTGGACGGAAATTCCACTTGGAAATTTCAAATGAGCGGTACC KI-homologous recombination fragment-3'arm : CTGCAGAAAAAATCCGCGAATCTGTAAAAAAACCCACTAATTTTCACCTCTTTTTCACACAAATCTCTCCGGTTTTTAGTACGATGCTCCGATATTCCGGCGGAATTTCGGAGCATCGTACTGATCACAATTTTTCCTCTTTTTTCTACGCAAAAATCAATAATTTACGCACAAAAACTTCAAACTTCCATGATTTTTTCCCACTTCTTTTTCAATTTTTCTGACATTTTTAGGTCCCAAAGTCCTTTTTTCCCCTGAAAAATTTCAGTTTTTCACATTTCTAATATATCCTACGCATTCCAATATTATTGATTTACATATACTTTTACTATCAAGATTTTTCAATCAATAAATGTTCAAATTTGAAATTTATGTTTAAAAGTTCAAGTACCCAAACCTTTTTTTTTTGAATTTATAATCGCTTTTGACACAAAATTTGTTTAGAAATTCAGATTCTACACAAAATTTGCAAAGTTTTGACACCCATATTGGCTAGGTTAGCATTGCTTTGACTTTCAGGCCGATTCGGGCCGATTTCAAGCCGCAATAGGCTTTTTTGGGAATCTAGTCCAAATAGGCGTTCATTACATATACATTCATAAATGGTTCGATTATCTCATTTTTTGTCTTCAGAATAACTGTTACGGGGCCTTTTAGGCCTGAAATCGGCTTGAAACGGCCTGAAAAACTCAAGTTTCCAAACCTGGTCAATATGGGGTTTAAGATTTTGCAAATTTTGCGTAAAATCCAAATTTTTCATTTTCGAAAGTCCTAGCTAGCCCCAAGTGTCACAAACGACGCTCCGAAATTCCGCTCGATAAAGTTTAAAAATTATATGATTAAAAAAAAAAAGTTTGTTTAAATCACCGACCACCATGTTATTCAAACGTTTTCACGTACAATCTAATCATATTACTAGT KI verification primers : TTX-1-CKIF: 5'-TCAACGCAAAGTTGTGTTTTTCT-3' TTX-1-CKIR: 5'-GGGGCACTTGAGGACAGCTA-3'