KI information : 	mNeonGreen knockin at N-terminus
sgRNA1 : 	TCGCTAGCTCATCTACAAAA
sgRNA2 : 	"TCTTGACTTCTTGGAATCAG
HND-1-NKIsg3: ATATCACTGACTTGAATCGG"
KI-homologous recombination fragment-5'arm : 	GCATGCGATGTCAGAAAACAATGCTTGCTTTTATCAAATATAAATGTAGATAAGCATTTTTTGAAATAATGGAACACAATCAACAAATATTCACAACTAAATCCCTTAATTTCTGTGCATCAATAATGCCAATGTCTCTACCGTATGATATCAATTGGCTCACTCTGTAATCTAAACAAAGTGTAATTGAGTCCGGCACGGGTTTGTCGGCATCTTTTCGAAGAGGGGTGATTGCGCGAAAGCCGTGCGTCGGGATGCCCAGACAGGGCGGCAAGCGGCTAGTTTTTGATGGCGGGGGATGGTCCGGATGGGAACTCGTATACCGAGAATTCATAAACATTTGTAGACTGTATCTGAACTGTGATTTACTGTGTTGATACTAGTGGATTCTTTAGAAAAGTATCTTAAACTCAAAAAAAAACTTTCTTATAGTCATATTAACTTATGTAGCAATAGTATTTGAATAAATTTTATGACGTCAGATAATAAGTTTCAATAAACATCGCAGCACTAATTGTAAGTGAATAATCCTGTTACTTTATTTGAGAATATAAAAATTATACTTATATTTTTGCCTCAAACTCTGATGCCATGCATATTTCACGGATAACCCATATTAAAAAGTCAAATATCGGAAGTCTATTTTTTTGAACATTTTTGGATAACCATGTACATATACACATCATCAATTTTACAACCCCTTGCCATTCCAGTTTTACAGCCTGTCTTTATACAAAAAATCTCCACCTCAACATAAGTATAATGTCAACAACAAAAACATATTCCAGGCCGGATAGTAGTCTACACAGATCCTCCCACCATCGTGAACCTTTCCTGCCCGCATCTATCCTCCCTTGCGGGGTCTGTCTTCTTGCTCCGCCCACCCGTACCCATCTTGCGTACCTCATTCCTCCGATTCAAGTCAGTGATATATCGCTAGCTCATCTACAAAGGTACC 
KI-homologous recombination fragment-3'arm : 	CTGCAGATGGTCAAATCAACAACTGCTGGAAACAATGCGGTTTCTTCATTAGAATCTACTGACTCCAAGAAGTCAAGAAAGGAAAAGTCTCGCGAGAAGGAGCATCGTCGTGCTCAATGTATCAACTCGGCCTTTGAGATTCTTCAGCAGTAAGTTGTTAACATTTCTTTTCCCTAAACAAATAGCTAACTTAATCTTATTTTCAGACATATTCCATACCTCAAATCGGAAGAGAGAAAGTCGCTGCCAAAGATCAAAACGCTTCGTCTTGCCATGCAGTACATTGATCATTTGAAAAAATTGCTGGGAGGAAATGAGATGGTATGTCGAATCATAATGTTTGTAACTGTAGTAATACTTTTTCGTACCATCAGTCAAAATGATCGTGTTTTGAATTATCTACTGTTTCAAATATCAAATTTCTTCAAATTCCTAACTCTTGTACGTTTTTTCAGCTCGAAACCGATTGCAACGAATCTCGCCCACTCACTCACTCAGATTTCCGTGTCAATATCTCGAATGAGATTAGAATTCGGAACAGTTACCGAGAGAGAGCCCACAATCAGGAGTTGGATGAAGCAACTGTGAAGAGAATTTTGGCTCGCGAAGATCAACGCCGTCGCTGTAGCTCAGTTCCGGAGGGAATACAAAGTAAGATCATAAAAATAGAGACATGGAAAAACAATTGAAATTTTCAGGATATACTCCTTACCAGAGCCGTCAATTCGGACCGATTTCGAACAATGTGTGCAACTTTCGCCACGTAGATGCTAACCAATACAACACCAACTTCATGGAATATCAAACACAAATGGTCCAGGTCGCCAACTTCCCAAATCAGTTCTCGCATGAGTATTACAACTATGGCACATTCCCAGTTATTGATCCAACTTCATTGGAGAACAGTAATCCAATTTTGCAATAACATTGTCTCATGTTATGTTACTCACGGTTTCCTTAATTGATCTCTATATTTCGACACTAGT 
KI verification primers : 	HND-1-NKIF: 5'-TATAAGGCGTTTTCTCTACTTAGCAAG-3' HND-1-NKIR: 5'-TAGGATTAGAGGCCACCAGGG-3'