KI information : mNeonGreen knockin at C-terminus sgRNA1 : TGAAGTTCTCTGGTGAAAGT sgRNA2 : ATTATTATAAAGATCAATTG KI-homologous recombination fragment-5'arm : GCATGCAATGCACAATATTTCACGACAGAAGAATAGTGTTGTTTTAATTTATTTTTTTCTAATTAGTTTAAAGTTAATTTTTCTTGCATCTGCCGTGATGTCGCAATTCCCAGAAGTTTTAGACAATAATGTAAGTAATACAATTTAAAATTTTCGACTTCCCAGCAAATGTTTCATTTATTTCAGCTTTTACACGCCGAAAACAACGAAACTGCCGAATACATACAAAATGATGGGACAAATCAATCAGAAGTGTTCATGGAACATCCCTACACGGTGAATAAAATCAACTCGTTTTAAAAACTTATTTATTTTATTTCATTTTAGCCGAACATGCATCCAATAAATATGCCTTCTATTGTAGAAGGTGCGGTTCATCCTTATAATAATTTAATCCATCACAATGATGCGATACCACCGGCAAAGTACGCCTCGATGAGACAAATGACAGAAGATTTCTGGCGTGAAAAAAAACAGAAAATGACTGAGATTTCTGAAGAGGATATGCTGAACAAGTCGAAAAACATGAGCGTTCCCATGGCTAGAGTGAAAAAGATTATGCGAATAGACGACGATGTTCGCAACTTTGTGAGTAGTGTCATTTTCCAGCCGATCTTCATACACTTTTTACAGATGATCGCGTCTGATGCTCCGATATTTATGGCTCAAGCAGCCGAGTTTTTCATTGAAGAAATGACCGCAATGGGCTGGCAATACGTGTCAGAAGCAAGGCGACGAATTCTTCAAAAAGCCGACATAGCAAGCGCAGTGCAAAAAAGTGATCAGTTCGATTTTCTCATCGACTTTCTACCTCCGAAAACGGTGCCAACAACTTCCACAAACGGCCCAGGCCATATGAGTGAAGACAGTTTCCAAGGTTTTCGGTTTTTTTCCTAAATATGATTTTTGTGCAATTTTTAACCTTTTATAGATCCCAACATGCATTCTGACTTTCATCAGAGAACTTCAAATTCTTCAGTCAACCGATCTCACCACAATGGTACC KI-homologous recombination fragment-3'arm : CTGCAGTCTTTATAATAATATTTTCTTGCTTATATTATGTATTAAAATTAAGTTCGTGGCGCCTGGCGCCCTTTAGTTCGTGGCGCCTGGCGCCCTTTAAAAGCTTGAATTTATACCTTTTTTGTCTAAAAATACTGTGTTTCATATGCACATTCATATATTTTCTTATTTCTTTGTTTTTCATTTTACAGGCTTTTACTTTGAAATCTTTAAATTTGAGGCGAAATGTGGTTGTCAAGTGAGGAGCTGAGTGCGAAGAAGACGGCTTGCCACGTGAGAAATGTGGAATCCAACAAAGAATTTGCAGATTCTGATATCGTTGCCTCATTCGTGCACAATCGGCCAAAAGATTTGAAAAACGTTTGTTCTAAAGCACCTTCTTTCAAATGAAAATAAAAATCCATTTTAGCTGACATTCGAAAGACACGGAAAAGGAGGACGTAAAAAAGCGCCAATATTCAGCGTAAAAGGAGATATTAGCGAGCATGTTGTAGAAGTTGGGGCAGATGTTAATTGTCTTGATGAGGGATTCGATTTTGCTGTTGCTTTGGTTGATAAGGTAATTTTGAGTGTATTTTAGCTAACAAATTCACTCTTTCAAAGGCTGAGTACCGAAACCTGAAAAATATTTTGGTACTACTTCTAAATCACCCCGCCACTTTTTTTCTTTTTTTTCAAATAAAAACGTGTGGAGTTTTTGTATTTTTAGGCTTAGGAAATAGCCATTTCTAAGCCTAAAGATGGAAAATGGACATCACGTTTTATTAAAATGAATAATATTAAAATGAGGGGAAATAATACACAAATAAAAAAAGTGGCGGGGTGATTTAGGACTACTACCAATATTTTTTAATGACTCCAAATTTTCCCCTGATTCCGAAAATCTATCAAAAAAAAATTTCACTGACTTTGGATTTCAGCTTGAAATCCCCGAATTTCATTTGTGAACCCTTGACTAGT KI verification primers : NFYC-1-CKIF: 5'-AAAATCAGAATTGAAATTGTCATCG-3' NFYC-1-CKIR: 5'-CGCCATGTGTCGATTTACGA-3' NFYC-1-CKIF2: 5'-AGGTTCTCATGAGGAAAGAACGTC-3' NFYC-1-CKIR2: 5'-TTGTGCACCACCCATGAATAAC-3'