KI information : mNeonGreen knockin at C-terminus sgRNA1 : TCCGCTCAACGCTCGTCTTC sgRNA2 : "ATTATGGTCCATATGTATTT Y73E7A.1-CKIsg3: TTTGCTTCAATTGCGACAGG Y73E7A.1-CKIsg4: GCCAGAAGACGAGCGTTGAG" KI-homologous recombination fragment-5'arm : GCATGCGTGTCTTATGGATAAGAAATCGAAAACAATGTAAAATAAATGATACATATTCTGCAATCTTATGTAGTTTTCGAGTTATACAGATTTTAACAACTCAAGTGAATCAAAACCGAAAGAGCACAAAATGTCAGTACTTCAGGTCGCAAAGCGACCCAGGTCGCTAGGTCGCAGGTCGCAAAGCGACCTAGGTCGCGATGCGACCTAAATGAAAACAGCGACCTAGCGACCTAGGTCGCACTCTTGCCACCCCTATGTGGGAGTGCCTCATTTCGGCTTGATCTACGTTGATCTACAAAGAATGCGGGCGAAGAGACGCAGAGTTCTCAACTGATTTCGCATGGTTAAGAACGTGCTGACGTCACATATTTTTGGGCAAAAAATTCCCGCATTTTTTGTAGATCAAACCGTAATGGGACAACATTTTTCGAATTTTTTTCAGAATTTTAGATTTTCACGCGATTTTTGATCTACTAAATGTTAATTATTAAAAATAATTTTCTATGCAATTTTCAGCGATTTTTTTTTCAAAATTTTTGAAAATTAAACTCAATAGACTTTTCATTAAAATTTATTATTACAATTTTCTATTTCTAAAAAAAATATTTTTGTTCAATTTTAAAATAATTTTTGAAAAAAAACATCGAAAATTCCGAAATTCGAGAATTTTCTGCGGTTTTCTGCGATTTTTCCATTTTTTTCTAAATTAAGAGTAAAAAAAACATAAAATTTCAGTAAATTTCCCCAAAAATCCCTTAAATTCCCCATTTTCCAGGCCTTCGAAGAAGCTCGGCTCCCCGAGCTAAAACTGAATCATCCAACGTTTCGTCTGTCGCAATTGAAGCAAATTCTCAAGAAAGAATGGCAAAAATCGCCGGAAAATCCGCTCAACGCTCGCCTTCTCGCTCTCAACTCCGGTACC KI-homologous recombination fragment-3'arm : CTGCAGATACATATGGACCATAATTTCCTATTTGCCCCTCGGTTTCGGTTTCTCGTCAATTTTTTTTCTTGGAATTTTATGGGTTTCTGTGTAGTTTTCCTGTTGTGAAAATGCATAAATAAATATATATAGAATGTTTGAAATATAGCATTTTTAGCAAGCATTTTAGTTCTGGAGCAGGGGTGGGCGGCAAATTGAGTTGTCGACAAATTCGGCAAATTGCCGGTTTGCCGATTTGCCGGAAATTTTCAATTCCGGCAATTTGTCGATTTGCCGATTTGCCGGAAATTTTCATTTTCGGCAAATTGCCGATTTGCCGGAAAATTTCAATTCCGGCAGTTTGTCGATTTACCGGAAATTTTCAATCGATCTTACAGGAAACAGTGAACGCTTACGAACTCATTTCGTGTCTCATTTCGGTTTGATCTACGTAGATCTACAAAAAAAGCGGGAGTTCTCAACTGATTTCGTATGGTTAAGAACGTTCTGGCGTCACATTTTTTGGGCAATATATTCCCGCATTTTTTGTAGATCAAACCGTAATGGGACAACCTGACACCACGTGAATATTCAGAATGTTTATTTTTTCTAATATTATAGAAAAAACACGATCGCACAAAAATAAACAACGACCCAACCAACGACACTCCGCTGTTTTAAGCCTCGCGGCCGACGCATTTCTGTGCAAATGAAATCGTGGGCGGGCCTTTAAAATAGCGGAGTGTCGTTGAAAAAAAAACAAAATTGGAAAAAAAATTTCGCGCTGAAATATTTACAAAAAATTTTGGCGCTAATTTCAAATCTACCGAGAGAATTTGCACAAAATTCAAATTTGTATTTTTAAGACGAATTTTAAAATAAAAAAAAAATTTGGCGCAAAAATATTTTCACGCGGTTCTAGCTTCCCTCATAAACTGAAATGGAATAGTTACTAGT KI verification primers : Y73E7A.1-CKIF: 5'-CACAGAACAAAGCATTGATTTCTACA-3' Y73E7A.1-CKIR: 5'-TAGTTTTACAACTAAAATCGAGCCG-3' Y73E7A.1-CKIF2: 5'-AACAATTTTAGTCAGCAGCAAATCC-3' Y73E7A.1-CKIR2: 5'-TTCGTAGTTCTGGCCTTTCTCATC-3'