KI information : mNeonGreen knockin at N-terminus sgRNA1 : AAATGTCACTTCTCGACCCT sgRNA2 : AGTCGGATCCAGATAAAAAG KI-homologous recombination fragment-5'arm : GCATGCGAAACTATAAACAGAACTAATTTGAGAAAAGCGACAAAAATATGTATTCATTCAGAAAAAAAGGAAACAGAGTGTTTACAACAATGATTGAAATTATTTGAAATTAAATAAAAAACAATCACCGTATTTCTACAGGTTACTGAAATCGTGATTCGTAACAAAAATTTACCGTAACTTCTATAACTTTTTTTTGCCGAAAAATACGTTGAGTAACCTTTTGTCGATTTTGTGAAAAACAAACCGACTAAGCTTATATATGGAGATTAGTAAGATGGTCTTTCTGCAAGTAAGTGAAAAACTGTGAAAATGAAACTTTAACATTTCGAGCACCAACCTGAAAATTAGAGTTCGATATCCAGCTTTGGCAGAATAATATTCCATATTGTTTATTTCCAAGTTTGACAATTCATCAAATGTTTCCCTAGAACCACCGTTTGACAGGTTGCTCCAATTAGCCGCAGTGTGCAGCTATTCCCAACCATCTTCCCCCATCTCATTTTCGCCATCATTTTCCCCCGCATCATCTGAGATGTGCAAAATGAAAGGTCAAGTTATAGATTTCGTGTTATTAAATACAAATAATTGACTTCTCATGTGGCGTTGGATCTCGAAAATCGAGTGTAATTGTCATGACCATGTTTAGGAGGGCGGTGGTGGTAGCTGCTGCTGCATCATCCTTCCCAATGTCAACGCACCCTCTTCTCTCACCCAAATTGCCAGGCCGTCGCCCCGCCCACTCTCGCACATTTAACTTGTCTTATAGTCACCCCTCATCGTCCGACAATAACCAATCATCGGTGCCTTCAACAGTGTCGCATTCCCGACTTTCGAACGTGCAAAGCATTCGTTTAACACTTTTCTACAGTACCACTCACTTCCCAAAACC GGT KI-homologous recombination fragment-3'arm : CTGCAGATGTCACTTCTCGATCCTCGACAGTTTCTGCTTCCTGCTTTTTATCTAGATCCGACTACACAAGCACTTCTAGCTCAAGCAGCTTCAACTTCCCCGTGCAACAAAATTTCGTCTTCTTCTTCATTCCGCATTTCTGACATTCTCGAGCAATCCCCAAACAATTCATCCCATTCAAACGATCACGGTAAGTCATTACACCTGCAATTGATTCCAAAATTCCAAACATTCAAAACGACAGCCATTAAAAGTTTTGTGCGCGGCGCGTTACCCATCAAAAATTTACTCAACCATGCTCTCGATTTTCTCCTCTCAATGGTGACTACCCGGAAATGATAGTTTGAATGTAGGGGGGGGGGGGGGTAAAAGGATTAATGCCTTAATGAACATATGAATACAGTTCTCTTCCGGGGTCAATCTTTTGTTTTGTTCTTTTTGTGCATCAATAACCATCAACTTTCAGACCCATCGCCACAATCAATTAAATCTGATTTTTCAACTTCGCCAAGAGCAAGCAGCCCCGGCGGCGATCGAATGGGTAGTCCAGGCAGTTGCAAAAAATCTCGGAAGGCAAGAACCATCTTCACTGACAAGCAGTTGCAAGAGTTGGAGAATACATTTGAAAAGCAAAAGTATCTATCAGTCCAGGACAGGATGGATTTGGCGCACAGAATGGGTCTCACTGATACTCAAGTCAAGACGTGGTACCAAAATAGAAGGTTAGTAATAACGTCTTTCAATTATCGTTGGAATTATCGTTGGAATTATCGTTGGTAGCAGAGTCAATTCTTAACATACATATGTACATTCTCAAATTAATAACAAGCGTAGCCGCCAATTGAGCCCACCCTTGTAGATTACTACACCAACCACGCTCACATTTCCTAATTATTGTTCAAGGCGGCGGTGTTCTATGTGAATAACTAGT KI verification primers : CEH-30-NKIF: 5'-TAATATCGGCAAGTGGCCTTATTC-3' CEH-30-NKIR: 5'-AGATTGTGAATGCATGGATACATTTC-3'