KI information : GFP knockin at C-terminus sgRNA1 : GAGATTTTATGTGATAAAAG sgRNA2 : "SKN-1-CKIsg1: TCTTTTATCACATAAAATCT SKN-1-CKIsg1: GAGATTTTATGTGATAAAAG" KI-homologous recombination fragment-5'arm : GTCGACTTAACCTAGAAGCGGCTTAAAATATTTCAAGATATAAGAGAGTTTGAAACCCATACCTTTCAGTTTTCTTATAATTATAAAAATTTCCAGTGTTCCCAACATCCAACTACGCCTACATTGGAATGCAAAACGACAGTCTTCAAGCAGTTGTATCAAATGGACAGATTGACTATGATCATTCCTATCAATCCACTGGTCAGACTCCACTGTCTCCTCTCATCATTGGATCTTCAGGACGTCAACAGCAGACTCAAACGAGCCCAGGAAGCGTCACAGTGACTGCAACAGCTACTCAATCGTTGTTCGATCCATATCACTCACAGAGACACTCGTTTAGTGATTGCACTACGTAAGTTGCTCAGTCTGAAAGTCTAGAAAGTCTCTTGGGATCCCATTCAGCTTATTAGTAAAAGTGCACTTGCTCAAATAGGGCAAGTGGGTACCCATGGGAACAGTTTTCAAAGTACTTTTATGCAAAAGTTCAATAATTACATAATTTCAGTGATTCGTCATCAACGTGCTCTCGCCTCTCTTCGGAATCTCCACGATACACGTCAGAGAGCTCAACCGGAACTCACGAGTCTCGTTTCTACGGAAAGTTGGCTCCATCCAGTGGATCACGCTACCAACGATCATCGTCTCCACGTTCATCACAATCTTCGATTAAGATCGCGAGAGTTGTTCCACTGGCCAGCGGACAACGGAAGCGTGGACGTCAATCCAAGGATGAGCAGCTCGCCAGTGACAACGAGCTTCCAGTGTCGGCGTTCCAGATTTCGGAGATGTCATTAAGCGAGTTGCAACAAGTGTTGAAGAACGAGAGTCTCAGCGAGTATCAAAGACAGTTGATTCGCAAGATTCGTCGACGCGGTAAGCATCATTATTGATGACAGGATCGAAATGATTTTTTTTGTAATTTCAGGAAAGAACAAGGTTGCTGCCCGCACTTGCCGTCAAAGACGCACGGATCGTCACGACAAGATGTCCCATTACATCGTTACC KI-homologous recombination fragment-3'arm : CTGCAGTCGAAACCTTATTAAAGCCACATAATTAAAGATAATTAATTCCGCCACAATAATCGTTTTTTTCTTCTTTGCCGTGTCTCATTTCATTTTGATCTACTCTTTCCTCCCTTCGGATTCTTTGATTTCCCAGTGAAATACCTCACCCACTTCAATCCCCACAAAGTGAGCAACCCCTATCTTGCAACAGTTTTATCATCTCTTCATCATACCCAGTTTGATAATTTATTATCTGATCCCCATCCCCTTGTCGCCTCTCATTAGTATCCTAGTTTTTCATTTGAGCCCGGAGCTCAGACTACATCTCCGAATCATCATACAAATAGATAGAAACGGGTCTCGTGACGAAAGAATACGTGCACCACACGACCCCCCCATCCTGTTCACCCCCATACACCTGAAAAATATGATCTTTACAGTTATTTCTATTATATCCTCAAATCTCTCGTAATATCGTATCAATTTCCTCTTCTTTTTTGTCATTTTCAATTTTTCTCAAATTTCTCAGATCTATTCTTTTTCTTGTATTTTTGGAACTTGTATCCCTCCTCCATCCCCAGACTTCCCCTTCCCAGTTACTCTTGTACATTTTCATATATGTCCATATATCGTTTGAATCTCTCATTTATGGAAATAAATTTGAAAAAATCAATCATTGAAAAGCAAAAATTTCATTCAAATAAAATGATGTTGGTGAATTTAACACAGAAAACAGGGGGACTTGATTTATAAATATAGACAAAACGAGAATTAAGCATCGGCCATCTCAACAGCGGGCTCCGGAGCAGATGGAGAAGGACCAGCCTTCTCGGGTTCAGTTGCCACGGCGATATCCATTGGAGATCCTGCAGAAGCATGGGAACATCCACAGCCACCACCAGCTTGGCGAGCAAACAATAGTTGTTTCACGAGATGAGAGATTCTTCCAGTTTCAAGTTCATCTCCGACTGACTAGT KI verification primers : SKN-1-CKIR: 5'-CGAGACCCGTTTCTATCTATTTGTAT-3' SKN-1-CKInF: 5'-AGACATTCCAACATTTCCTCCTT-3'