https://tulab.genetics.ac.cn/author/herong-zhao/ Herong Zhao Hugo Blox Builder (https://hugoblox.com)en-usMon, 23 Mar 2020 00:00:00 +0000 https://tulab.genetics.ac.cn/author/herong-zhao/avatar_hu_6394a1e76061e90e.jpg https://tulab.genetics.ac.cn/author/herong-zhao/ https://tulab.genetics.ac.cn/publications/2020-genomebiol/ Mon, 23 Mar 2020 00:00:00 +0000 https://tulab.genetics.ac.cn/publications/2020-genomebiol/ <!-- <div class="alert alert-note"> <div> Click the <em>Cite</em> button above to demo the feature to enable visitors to import publication metadata into their reference management software. </div> </div> <div class="alert alert-note"> <div> Create your slides in Markdown - click the <em>Slides</em> button to check out the example. </div> </div> Add the publication's **full text** or **supplementary notes** here. You can use rich formatting such as including [code, math, and images](https://docs.hugoblox.com/content/writing-markdown-latex/). --> <p>Abstract: Many differential gene expression analyses are conducted with an inadequate number of biological replicates. We describe an easy and effective RNA-seq approach using molecular barcoding to enable profiling of a large number of replicates simultaneously. This approach significantly improves the performance of differential gene expression analysis. Using this approach in medaka (Oryzias latipes), we discover novel genes with sexually dimorphic expression and genes necessary for germ cell development. Our results also demonstrate why the common practice of using only three replicates in differential gene expression analysis should be abandoned.</p>