NHR-76

WormBase ID: WBGene00015497

WormBase description: Is predicted to have DNA-binding transcription factor activity and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in positive regulation of fatty acid beta-oxidation by octopamine signaling pathway; positive regulation of fatty acid beta-oxidation by serotonin receptor signaling pathway; and positive regulation of transcription by RNA polymerase II. Is expressed in several structures, including body wall musculature; excretory gland cell; intestine; pharynx; and vulval muscle.

Reporter verification: Whole-genome sequencing indicated that the GFP tags another TF, NHR-76 rather than F22D6.2
Primers used to verify fosmid reporter: 5'-ATTCATTGACCATTCATCCCTA-3'

Strain name: SYS558

Cross: OP399 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs399 (nhr-76::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Expressed after bean stage

Lineage expression pattern

350 stage


600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: ETS-5, MDL-1, NHR-102

Targeted by: CEH-31, ZIP-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: ETS-5, MDL-1

Targeted by: CEH-31