Single-Cell Protein Expression Atlas of Transcription Factors

ETS-5

WormBase description: Is an ortholog of human FEV (FEV transcription factor, ETS family member). Is predicted to have DNA-binding transcription factor activity, RNA polymerase II-specific. Is expressed in nervous system. Human ortholog(s) of this gene are implicated in acute myeloid leukemia.

KI information: mNeonGreen knockin at C-terminus

sgRNA1: TGCCGTCATCGTATTAAACG

sgRNA2: GTGTATGTAAGCGATGATTG

5'HR: GCATGCTAATACATATAGGAAATAGGAATGTAACACGTAGTTATGGTTAAGAGTAGTGTATATAGTCAAATTTTTTTATTGCTTTACGAGACTCAAAATTGTCTAAAAATACCGCATTTCGTAATGAAACTTCTCGAAAACGTCTCAAAAAATAAGTTATGGCTGCTGAAAAATTGCCCTGAAAGTAGTTATAACTTGAAATTTGACCGACAATGTGGCAGCATCCTATTAATTTTAGTTACCGTTATTTTTATAATTTTGCCTGCAATACTTGTTTTTGACTGTGTAAACGTTCTAGATTAAAAAAAAATGGTAGGTCATAATATTTTCCACTCACAAGAGTTCGAATATTTCCGAACTCTTGTGAGTATAACTTACGTTAAAATCGTGAAGAAGTTACCAAAATTGTTCATAATAGGCCAGGCAAAAAAATTAGTGCAAGACTATTAGAGGTTGTAATACTAATATAGGGAATAGAGTAATCCATTGAATGAATCATTACTCAAATTATTACATACACAACTAAATATCTTAATTTTTCAGGTACAAGGAAAGCGTTACGCGTATAAGTTCGATTTCCAAGGACTTGCTCAAGCTTGTCAGAGTGCAATTTTGACAAATGGTGGAAACCCCAACGGAGATCTGTCATCAACTGTTCATTCACTATCACCATACACAAATCGTAAGCTTTATCATTCTCAAGACCTTGAAATGTTTTATTCATTTTTTTTGAGAAGTGCTTCCAATTGGTGTGACCAGCCGATTATCGACTTCAATGTCTTCATATCATTCAATACTTTCCTCAACTTCGTCTACCTCTTCCAATCAGATAATTCCACCATCGGTAATTCAGAGATTTCCATGCAATGATTTTAAATTTTAAATTTTCAGACTGCGACATACTGGTCGACTCCTCAGTCGTCTCTCACATACACCGGAATGCCGTCATCGTATGGTACC

3'HR: CTGCAGACGCGGTCTGTATTTGAAGTACATATTTTGAGAAAAAAAAAAGTAACCATAATATTTATACCTTATTTGTCTATACTCATTATTTTTGTATCAAAATTACTTTATAAATGACAAATTATTTTTATACTATATTTTTATATTTTCCATGAAAAAAACACTTAAAGTATAACATCGCGATGTGATCGAAAAAAAACTATTTTTTCGAACAACATTGCAAAAATAAAAACCAGTTGGCGTAGATTCCGCACACGCAACTTTAATTGGTTTTGACAGTCGCTTCGATAATACATACGTGATTGAAAATGGTACAGTCTATCTCTTTTTTGTTGATAGTTTTTGATTGGTGCGCAGCGCACGTAAAGATGTGAATTATCAACAAATTAGTCTTTAGGTCAAAAGTGTGTTTATTGGCTTGGTTTTGAAAAAAATTGTTTCAGCATGACTGGAGAAAATTACTGAAGAGAATATGTAAGGTGTAGACGCCACGACAAAAAGTTTCGAATTTCATCTCGTGTTCCATCGGGTCAGAATGTTTGTTTTGAAATGACTTGAAACACAAAATATAACCAAAAATGATGTATATTCCTGGAAAATGTCGAAATACTTGGGTTTTTTTCATTATTTCTAAAGATCAAATAAAAAGAAGCAATATTTTATAATGGGTATAATATTTTCAGGCTCGTTTTAGATCATACATTGCATGAAAAAGGGAAAATACTAGGCTGCATGTTAAAGTTTAGACAAGATTTTAAGAAATAACCTTAACACAATTTGAGTGCACATTCTATACAAAAACAGTTTTTAGTCCGATTTGCAGCGAACGGAAATACAGGTCAGCGATGGACCACTAATTAAAAGGCTTTTCTAAATGTTAATTACTTCGGTCACTAGT

KI verification primers: ETS-5-CKIF: 5'-TGTAATCAAGGACTTCCAAAAAGGT-3' ETS-5-CKIR: 5'-AGAGTTCTAGCCTCAAATGGGTG-3'

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Strain name: SYS643

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; ets-5 (devKi200 [ets-5::mNeonGreen]) X

Expression pattern: Expressed after bean stage

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NA

Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: AHR-1, ALY-1, BCL-11, BED-3, CEH-13, CEH-14, CEH-18, CEH-19, CEH-2, CEH-24, CEH-31, CEH-34, CEH-36, CEH-39, CEH-43, CEH-48, CEH-6, CEH-83, CEH-9, CHE-1, CND-1, COG-1, DMD-4, DVE-1, ELT-2, ELT-3, ELT-4, ETS-4, ETS-7, EYG-1, FAX-1, FKH-10, FKH-6, GMEB-2, HAM-2, HLH-1, HLH-15, HLH-30, HLH-8, HMBX-1, HMG-4, HND-1, K09A11.1, LIM-6, LIN-11, LIN-13, LIN-32, LSY-2, M03D4.4, MEC-3, MED-1, MEP-1, MLS-2, NFYA-1, NHR-12, NHR-232, NHR-76, NPAX-4, ODD-2, PAG-3, PAX-1, PHA-2, PHA-4, POP-1, PQM-1, PROS-1, REF-2, RNT-1, SEM-2, SNAI-1, SOX-4, SPTF-1, SYD-9, TBX-2, TBX-7, TBX-8, TTX-3, UNC-120, UNC-3, UNC-39, UNC-42, UNC-55, UNC-62, UNC-86, ZTF-11

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data