LIN-22

WormBase ID: WBGene00003008

WormBase description: Is an ortholog of human HES1 (hes family bHLH transcription factor 1). Is predicted to have DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including anterior/posterior pattern specification; epidermal cell fate specification; and nematode male tail tip morphogenesis. Is expressed in seam cell.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: GATGCAAGAAGATTGTGAGT

sgRNA2: GATACTGAAATTGAATCCGA

5'HR: GCATGCGCGTGTCTTTACCCCCACTTTTTCCTCTTTCGAAATATAAATTTCAAATTTGATTGACAACAGAAACTTTAAGTTTTATTGGGTTTCTTCATTTACAAGATTTGCCCTATAATACCTATGATACTCAAATTTTGCCGTGCATTCACAAAACGCCTGCCTACGCGCCTACGAGGCATACTCAGATCAAGCCAAACAGTTGTCAACTAGGGCTACTAGGCCTTGGGCTTAGGCTATTCTTTGGACTTGTTGCTTTTTTCGAGCAGTTTTCATGGGCGGACTTTGGAGAATGTCAGTTTTTTTGTTTTTCTGAATTATTTCAGGGGTTCAGCCTAAGCTTAAGCCTAATCCTAAGCCTAAGCCCAAGCCTAAACTAAAAGTAAGCCTAAACCTAAGCCTAATCAAGATAATAAAAGTAAAAAAAACGCGATTCTGCAGAAATCTAACTTGCTCAAAAAAGGTGAAAAGCATAGTCCTCCGCCTAAGCCTACGGCTTAGTCTAAGCTTCAGCCTAAACCTAAGCTTAAGCCTAAGCCCTGTTTGGAGTGTCTCATTTTTCGTTCTATTTTCCTTTTTTGAATTTTTGAATTTTCTGGGATTTCTTTTTCTCTTTTCCTCTCTTTCTCAGCCCAAACCTAAGCCTAAGTCCAAGCCTAAGATAAAGCCTAATCCTAAGCTAGGCCGTCAACTAGGCCACCATCAAAGCTTTCTAGGCAGGCAGTTGTATGAGCCTAGTTAATTAAAATAGTTGGAAATTAAATTTGAAAATTAAAAGAGCTGAATAACATAAAGTCAGAAAAACTACCAATTACATAGTTTTACTGTTCAATAACTTATTTGGTGATTCTATTTTTGCTCTTTTTTATATTCATTCACTGTATCTAATACTTTTTTTTTGACGTTACTAATATTATTACAATTATTACAGAAGGTACC

3'HR: CTGCAGATGACGTCATTCCTGTGCTCCGATACCGAGATTGAGTCTGATGGTGGAATCTCCAGATGTAAGAAGATTGTGAGTTGCTTTTTTTCTGTAATTATTCAAACTTTTGTAATTTCACAGCTGTCCATAATTTTGAAAATCGGCTTAATTTAATTTTCAACATTTTCAGAAAAACAAACCTCTAATGGAGAAGAAACGGAGAGCTCGAATAAACAAGTCACTGTCACAACTAAAACAAATTTTGATTCAAGATGAGCATAAGGTATCGATTTTTCTCAATTATTTATACCTCCGAAAAAACAAGTTTATAAAATTGATAATGGAGATTTTCAAATTTTTAAAATTTTAAGACGAAATTTTGGTAAATTGTCAAGTTTTTAAAAAAGTGAACTTTTTTAGCATTGCTTCAGATCAGGGGCCTCGGCAAGTTGCCGGAATTAAAAATTTTCGCCAAATTGTGGTTTTGCACTTCTTTTTGGAAATTTCAGAATTTCAATTTTAATCGGCAAAATTGTACGCATCCTGTGAATCTTGTTACATCTATTTTAAAAAGTAGGCAATTCTATAAAAGAAAATGGGAAAAATATTTCAAAAAGGCACAGTTTTAAGTGTTTCCGTCTTATAAAAAATCCCTCTAAAATTTTCCGGCAAATTTTATATCCAAGTTGCCGGAACTGAAAAGTTCCGGTAAACCGGCAATGTGCCGAAAATGAAAATTTCTGGGAATTTGGCAAACCGGCAATTTGCCGAAAATGAAAATTTCCGAGGAACCGGCAATTTACCGAAAATGAAAATTTCTGGGAAATCGGCAAACCACCAATTTGCCGAATTGCCGAATTTGCCGGAAAAACGGCAATTGCTGAAAAATTTCGGCAAATTGTAGTTTTGCACCTTTTTCTTGGAAATTTCAGAATTTCAATTTTAATCGGCAAAGTTGTACGCATCATATGAATGTTCCTACACTAGT

KI verification primers: LIN-22-NKIF: 5'-CACCTCCTGATTCAAATGCTTCT-3' LIN-22-NKIR: 5'-TCTTCAGATATTTTAATAGAATTTGCTTATC-3'

Download

Strain name: SYS1050

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; lin-22 (devKi259 [mNeonGreen::lin-22]) IV

Expression pattern: Skin-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): RNT-1, CEH-16, NHR-23, CEH-40, GMEB-1, LIN-39, LIN-26, CEH-13, ELT-1, LIN-1, NHR-25, HAM-2, CEH-20, HBL-1, UNC-62, TLP-1, NFI-1, Y75B8A.6, CEH-43, CEH-74


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: RNT-1, ELT-1, UNC-62, CEH-36, COG-1, TBX-2, UNC-120, UNC-55, BLMP-1, LSY-2, MEP-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-36, LSY-2