CEH-16

WormBase ID: WBGene00000439

WormBase description: Is an ortholog of human EN2 (engrailed homeobox 2). Exhibits sequence-specific DNA binding activity. Is involved in several processes, including embryonic body morphogenesis; epidermal cell fate specification; and regulation of transcription by RNA polymerase II. Localizes to nucleus. Is expressed in several structures, including ABarpa; ABplaa; hyp5; retrovesicular ganglion neurons; and seam cell.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-ATTTCAGAACAAGCGTGCCA-3'

Strain name: SYS101

Cross: OP82 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs82 (ceh-16::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Tissue-specific (Pharynx and Skin)

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): LIN-22, RNT-1, CEH-2, NHR-23, GMEB-1, NHR-12, CEH-40, REF-1, PAX-1, LIN-26, AST-1, Y48G1C.6, EYG-1, DMD-5, ELT-1, HAM-2, LIN-1, MLS-2, CEH-45, NHR-25, CEH-13, BAZ-2, TTX-1, HBL-1, CEH-20, UNC-62, TLP-1, Y75B8A.6, CEBP-1, NFI-1, SPTF-1, F23B12.7, CEH-74, CRH-2, ELT-6


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ELT-1, UNC-62, ELT-2, LSY-2, NFYA-1, ZIP-8, BLMP-1, NHR-85

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ELT-1, UNC-62, ELT-2, LSY-2, NFYA-1, ZIP-8, BLMP-1, NHR-85