CRH-2

WormBase ID: WBGene00016162

WormBase description: Is an ortholog of human CREB3L1 (cAMP responsive element binding protein 3 like 1). Is predicted to have cAMP response element binding activity. Is expressed in body wall musculature; seam cell; spermatheca; and tail. Human ortholog(s) of this gene are implicated in achromatopsia 7 and osteogenesis imperfecta type 16.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-GAGTACATTGATGCCCTTGA-3'

Strain name: SYS274

Cross: OP425 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs425 (crh-2::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): PQM-1, RNT-1, PAX-1, PHA-2, EGL-13, DAF-19, MDL-1, LET-381, UNC-86, NHR-23, CEH-16, CEH-31, PAG-3, BLMP-1, Y56A3A.28, BAZ-2, LIN-26, LIN-39, CEH-8, Y48G1C.6, HAM-2, CEH-34, LIN-11, GMEB-1, TTX-1, VAB-3, HBL-1, TBX-2, TTX-3, CEH-48, UNC-42, CES-2, PROS-1, EGL-46, ATF-7, Y75B8A.6, EGL-44, ZAG-1, AST-1, UNC-39, NHR-12


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: RNT-1, CEH-31, HAM-2, CEH-34, TBX-2, UNC-42, PROS-1, ALY-1, BCL-11, CEH-18, CEH-2, CND-1, COG-1, ELT-1, ELT-2, ELT-3, HLH-1, HLH-30, HLH-8, HMG-4, K09A11.1, LSY-2, MED-1, MEP-1, MLS-2, NFYA-1, PHA-4, REF-2, SPTF-1, SYD-9, UNC-120, UNC-3, UNC-55, UNC-62, ZTF-11, PQM-1, EGL-13, MDL-1, UNC-86, BLMP-1, ALY-2, C16A3.4, CEH-82, DPL-1, DSC-1, EFL-1, FKH-10, FKH-8, FOS-1, IRX-1, LIN-13, MEF-2, NHR-85, PAX-3, SKN-1, SWSN-7, TLP-1, UNC-130

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: RNT-1, CEH-31, HAM-2, CEH-34, TBX-2, UNC-42, PROS-1, ALY-1, BCL-11, CEH-18, CEH-2, CND-1, COG-1, ELT-1, ELT-2, ELT-3, HLH-1, HLH-30, HLH-8, HMG-4, K09A11.1, LSY-2, MED-1, MEP-1, MLS-2, NFYA-1, PHA-4, REF-2, SPTF-1, SYD-9, UNC-120, UNC-3, UNC-55, UNC-62, ZTF-11, PQM-1, EGL-13, MDL-1, UNC-86, BLMP-1, ALY-2, C16A3.4, CEH-82, DPL-1, DSC-1, EFL-1, FKH-10, FKH-8, FOS-1, IRX-1, LIN-13, MEF-2, NHR-85, PAX-3, SKN-1, SWSN-7, TLP-1, UNC-130