WormBase ID: WBGene00001210
WormBase description: Is an ortholog of human INSM1 (INSM transcriptional repressor 1) and INSM2 (INSM transcriptional repressor 2). Exhibits RNA polymerase II transcription factor binding activity. Is involved in cell fate commitment; generation of neurons; and positive regulation of exit from mitosis. Localizes to nucleus. Is expressed in several structures, including QL.aa; QL.pp; QR.aa; QR.pp; and neurons.
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KI information: mNeonGreen knockin at N-terminus
sgRNA1: GTAGATGTTGTAGACGGCAC
sgRNA2: GTTTTGATTTCAGAAGGCCA
5'HR: GCATGCTAATTGTAGATCATAAAAATACATACCGTTATAAAATTACTGATATTTGTATCAAATATATTTGTACAGACTTATAGACTCTTTAGATTCTTTCCAGTGATTCTCTGAATTTAAAATATTATTTTTGCGGCAGTGGGGAAATTGTTAAAAACCACTCTTATGGTGCCAAAATTAGCAGATATTATAGTGCAACTTTTCAATTTTTTTTTGTTGTTTTTTTCACTTTTTGGAAGTCGTCGATCAAAAACAATTATACCTTCGAATTTTATAGTTTACTTATGTTTAAAATTAATATTTATCAAGCACTGAGAGGAATTCGAACATGCGGCATTGCTATAGTTTTCTCGAAAAGTGGCAAAAACTTAGTAATTGCCACTTTTCAAATGCCAATAAAAATTTCATTGATAAAATCTCAATCCAAAGAATTTCTGTGGAAGGAAAAATTCAACAGTGAACACAAATAAAATTTCACAATAGGTTACGAAATGCCCAATTTCAAGCTATTGAGAATTCATCAGTTGTTTTACTGAGATTTTCCCTTTTTCTTCTATCCCTCAAAAGTTACACAACTCAAAAATCGAATTTCTAGATTTCGTCACTCATCTATCAAATTTACCCATTGACAATCTTTATTTTTGACGAAAAAAACATTGAAATTTCCAATTTCACAAAATCTCGACCCAATTTCCATGTTAATTGCTCACTCGCTCCCCTTCTTGTAAGGTGTCTTCGGGGCGGAGACTCGGGAGCAGCAGCTGCCAGCCTTGTGCCTTCTCCCATATAAAAGGAGGTTAGTGTGTACGTGCCACGGTCAATTGAGGACACCAGACGGTCAGAGTCAGACACCTTTTCTTTTTTCGTTCACATCTACCGTAACCCATATCTTTTTTCGATCCATTTTATATCGTTTTGATTTCAGAAGGCCGGTACC
3'HR: CTGCAGATGGTGCCTATGAATGACTTTTGGGTCAAAGCAATATTATCATCGACGAATCCGAGTCCAGTTCCTTCCACTACATCTACCGTATCCAATGATGAAAATTTGGATAAAACGTTGGACTTTGACTGTTCCACGCAAACAGTGTTCCCTACACTTCCAATGTTCTGGAACCCAACGCTAGTTCAACAGATGCTTGCGCTCTACCAGATCCAGCAGCAGCAAATTCAGTTCAGCGCGAAGCTGGCACCTCAACCGGTTAGTGATAGGTGTGTCTTATCTAGACATTGATACCTTTTTTCTTAATTGAAAGATCAAAAATTTATACCAACTTCTACCTGTTCTACTGATGACTATAGGGGAATGTGATTTTCACTGCAAATAAGGCGATCATAGTATTCATAATAAGTTCTCCAGATCACTTGACACTATTATGTTCTTGAGGTATCTAAAAATGTGTTCTAAAAAATTGTTTTCAGCTTTTCCAAGAACCGACCATCCAAAAAGAGTTTCTCCCATTCCCCCATCAATCTCGAAAACGTCCTCTACCGATAGACCCGAAAAAGACAAAGCTTCGAAAACTGAATGAAGACACTGTGACCAGTTCTCCAGTCAGCGGTATGTTCATAAAGGTGAGTAAAGCTATCGGCTCTTTCTAAGCTTCTCCTTCTTTCCTCTATGAGCAAAAACAATTCGGCACCGATTGTCTTAATTAGACGTGATTAGCATATGAGAGCTTTTGAAAACGAAGGCACATAAGAGGTGCCGGAAAGCGGTGCGGCGCAGAAAAAAGAAGGAGCAAAAGCTTGGGTTAATTACCACCCGCTGACCAGCGACCGTCTGCCACTTTTGTCTTGCGGCATTCTCTGACAACCGTCTCAGATTGAGCGCTCGCTTGATTTATTGCTAATGACACCGAGAAAACGAACGGATAATAATATTTTTGTTGCAGGAGGAGGCGGATGTGAAATCTGTGGAGGAACTAGT
KI verification primers: EGL-46-NKIF: 5'-AACACATATTGTTCGCCAATTAGG-3' EGL-46-NKIR: 5'-TAACCAACCTCTACATATGCTGCTG-3' Download
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Strain name: SYS637 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; egl-46 (devKi194 [mNeonGreen::egl-46]) V
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Expression pattern: Neuronal-specific
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): AHA-1, DAF-19, UNC-3, CEH-48, UNC-42, NGN-1, ZAG-1, CEH-30, CND-1, EGL-44, VAB-3, LIN-32, TBX-2, HLH-3, ZTF-11, ELT-6, CES-2, HLH-2, CEH-36, CRH-2
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: UNC-42, CND-1, LIN-32, ZTF-11, CEH-36, CEH-31, CEH-43, COG-1, HMG-4, LSY-2, MEP-1, MLS-2, REF-2, SPTF-1, UNC-55, TBX-2, UNC-86 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: CND-1, LIN-32, CEH-36, HMG-4, LSY-2, MEP-1, REF-2, UNC-55, TBX-2, ZTF-11, UNC-86
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