WormBase ID: WBGene00001208
WormBase description: Is an ortholog of human TEAD1 (TEA domain transcription factor 1); TEAD3 (TEA domain transcription factor 3); and TEAD4 (TEA domain transcription factor 4). Exhibits RNA polymerase II transcription coactivator binding activity and RNA polymerase II transcription factor binding activity. Is involved in generation of neurons; positive regulation of exit from mitosis; and positive regulation of transcription by RNA polymerase II. Localizes to RNA polymerase II transcription factor complex. Is expressed in several structures, including anterior hypodermis; body wall musculature; ganglia; neurons; and pharynx. Human ortholog(s) of this gene are implicated in Sveinsson chorioretinal atrophy.
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KI information: mNeonGreen knockin at C-terminus
sgRNA1: TACAGGCTAATCGATGGAGG
sgRNA2: AAACAAAAAATATGGCAGTG
5'HR: GCATGCTATCCGAAATTACTTCGAGAATTGTTCGAGAAATCCGAGAAAAAGGATGTCTTCTTCCTTGCAAAGTGTTGGGCAAATATTAATGTTTCGGATGATGTACAGTAAGTTATATTGAATGTGATTGATAATTAGTTTTGACTCAAAGAAAACCTTTTAGAAACTGTCAATACGCAGTCGACTCGTTCTACTCAAGTCGTGAAAAATTCCAATTAAAGGTCTCAACAATGGCTTGCTCATTTGGAAACCAGGCAGTTGAAAAAATAGAGGTACGAATATTATGAGCTAAGCGGCAATGTACAATCGGTTGATTTTTCAGCAATACTTCCCAATCGAATTCGATGGATCATATTCGTTCATATTGAACAACTCCCCAATGTGTGACTATATGGTTAAGTTCATTGCCGAGCTGAAGAAGCTGAATGTAATAGAAACAATGAACAACGTGCTGGAGAATTTTACAGTTTTACAGGTAAGAATATTTGAAAATATTTAACCTATTCAGAGAGTTTGTCAGCGGCCATTTCTCCACCTCTAAAAATTCACAAAAACTTAAATCAAAGTTGAAAAATAGCTCTAGAAAATTTTGAAAAGTTTCATTTGTTTCATTAGAATTGAATAAATAATATGCACATTAATGAATAATATACTTTTTAAGTCGCTTTCAAAAATGTTCTCCGACTGAAAGCTTAAAATTCTGAACAATGTTTGAACAGTGTTATAATTTTTTAATTAGTTTTCTAACACATTCTGGGGTTTTAAAGAAAAGGAATACGGGGTTCTAATTTAACAAAAAAAAACTGAGGAAAAACTGGAAAGTACACATAATGAAAAGCATAATAATTTTCAGATTGTCACAAACAGCGAAACTGATGAATTATTAATGGTTCTCTGTTTTGTATTTGAAGTATCACAAGAACCAGAGCCATCATGTTCTGTCTACAGACTGATTGACGGAGGAGGCGATTCTGATGAAGGTACC
3'HR: CTGCAGATTTTGAAAAATCTCTTCCGACACTGCCATATTTTTTGTTTTTTTCTTAATTTATACACTCATTCATTCCTTTTCTTCGTTTTCTCCTTCTCTTCAAAAATCGGTCACACGAATTCATTTGAATTTCATTTGAAAAATAATAATAATATGTTCCAGTCATTCCCATTTTCTTATTTTCTCCATGTTTACCTGAATACTCAAAACCCAATTTCCCGTGATTTATTTTTGTACTTTTTTTCGTCTCATCATGTGCAACCAGAAGAAAACTCGTCGAGCTTCTCATATTTCTTGGAAATTCACAGAATCCATCAAAAAGTCTACAGTGTCTAGTACGAGTTGTTCTTTAATTTCTAATCCCTCACATTCATCCCCACACTTCTCCCTTTCAGATTCAGTTCCCCCCAATACTTGCAACCTCTTCTCCACACCTTCCACAAAATCTCAATTCTCGATTTTTTCAACCCAAGTTTCCGATTTGATTCAGTTCTGTTAGGCTCTTAGGTGTGTTTTGTTTCATTTTCTGTATTCTATTTTTGGACTTGTTTTCCCTTTAATTATCAGTTATTGAAAAAATGCATCAGATACCTAAACTTTTCAATTTTTTTAAAAACGTTTTGGGAGCAACTGGATTTTCAGAAAGAAACGTTTCATCAAAGCAACAGAAGACCCCGACAAAACGAAAGGCGGAGCTGCGATATTATAAACAATGGCACTGTACCGCGCGAGGTCATTCCAAATCTCAATCACTTTCCATAGAATGTCTGCTCCATCTCCTTCACCTTCTATTTCAAGATTAAATTCAAATTCAGATGAAACTTTCCGTATTGTGGTAAACTATCTGGTAACTTCGAACAAACTTGAGGAAACACGTGTCAAGTACACCAGAATTATTGATTCTCTAGTCCTGCAAATGGACTGCCTGAACTTGATTGCCACTTTGACAAAGACAAATCTTGTCGTCTTGCTAGTGATCACTAGT
KI verification primers: EGL-44-CKIF: 5'-ACTCGTGAAGATTGAGAATACACTGG-3' EGL-44-CKIR: 5'-ATTTCTCAGCGAGATAATGGTATTCT-3' EGL-44-CKIF2: 5'-CACCTGTGAAATCAGAAGTTGTAGAAG-3' EGL-44-CKIR2: 5'-TTACATGTTGGAACATCATTTGAGC-3' Download
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Strain name: SYS603 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; egl-44 (devKi175 [egl-44::mNeonGreen]) II
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Expression pattern: Lineage-specific, Neuronal-specific
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): AHA-1, LIM-4, TTX-3, UNC-3, EGL-46, CEH-48, CFI-1, DAF-19, UNC-42, LIN-11, ZAG-1, EGL-13, CEH-31, UNC-86, CEH-5, TTX-1, CEH-30, NGN-1, PAG-3, HLH-14, VAB-3, LIN-32, Y73E7A.1, TAB-1, CEH-32, SOX-2, DMD-5, ATF-7, TBX-2, NHR-67, CEH-6, Y56A3A.28, UNC-39, CES-2, HLH-2, HLH-3, CEH-27, ZTF-11, CRH-2
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: LIN-32, CEH-36, CND-1, ELT-1, ELT-2, FKH-6, HLH-1, HMG-4, MLS-2, PHA-4, REF-2, SPTF-1, UNC-55, UNC-62, ZIP-8, TBX-2, BLMP-1, C16A3.4, DPL-1, EFL-1, ELT-3, LIN-39, LSY-2, MDL-1, MEP-1, RNT-1, SKN-1, UNC-130 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: LIN-32, CEH-36, CND-1, ELT-1, ELT-2, FKH-6, HLH-1, HMG-4, MLS-2, PHA-4, SPTF-1, UNC-55, UNC-62, ZIP-8, TBX-2, BLMP-1, C16A3.4, EFL-1, ELT-3, LIN-39, LSY-2, MDL-1, MEP-1, RNT-1, UNC-130
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