Y56A3A.28

WormBase ID: WBGene00013240

WormBase description: Is an ortholog of several human genes including ZBTB43 (zinc finger and BTB domain containing 43); ZBTB5 (zinc finger and BTB domain containing 5); and ZNF367 (zinc finger protein 367). Is predicted to have nucleic acid binding activity.

KI information: mNeonGreen knockin at N-terminus

sgRNA1: AGTCCAGCTCCGTTCCTCTT

sgRNA2: TTATCTTCTCTACCTTATTC

5'HR: GCATGCCAAAAAATTCCGATTTTCTCTGAATTTACCTCCTTTTTCATCTAAAAATAGGAAATTTTCGCGGTTTTCTGATCATAAAATGTTCAATTGGCTCGAAAATACACAATTTTCAACGAAAAAAACCGATAAAAATGAATAAAAAGCTGAAAGCTGGCTCGTAAATCGGCACAAGACACAGCGCGCTTCAATGTCTGGCGCGCGTTTGAAAATCGTCGATTTTCTACAATAAAAGGGACTTTTTTTTCCAATTTTTCACATAGATATTTTAAACAAGGAGCAAATTTGGAGCAAAACCTGGAGAATCTTGTTTTTTTTTTTGGTTTTTAACTGAAAATTGACAATTTTACTACAATTTTGTGGGAAATTCTTGAATTTTTTAAAGTTTTTCTGGCAATTTTTGACATTTTCCACCAAAATTTAAATTAATTTCAAAGGGAAAAATTGATTGGTTTCAGACGTGGAAAATCGCAAAATTTTTGATTTTTGTGAAAAAAAAAACCTATTTTTTAGCGTATTTTAAAAATATTTCCACTAAACTCAAATTTAATTTTTTAAAAAATTTTAAAAAATTTCCAGACCGCCCATCCGTCTCCGCTGAGTGCTCGCAAGTGGTGGGGATGCAAATGCTTCTCGAAATGCAACACGGAGCTCGAAAATTCCGGTCGAAACCCGATTAATTGGGCGGATTTATGAATAAATTCTCCTGTCGAACACCCTGCGTCTCTTCATCTCACACACTCTCTCGCCACTCACTGAAGAACTCTGTTATTTATCGATTTTTCTATTTTCTCTTCATTTTTCCTCATTTTTTCCTCATTTTTTCGGTTTTTCATCATAAAAATTCGATTTTCTGGCCATTTTTTTGTTCAATTTCCAATTTTTCATTCGTTTCAACGATTTTTAAATGCTTTTCACGGAAATTGCAGCGAAAAATTGTTTTTTTAACAGAAAAAAAGAGGTACC

3'HR: CTGCAGATGCGGCGACAAAAAACTGAAGAAGAGCGGAGCTGGACTTCAATTTCTCGGCATTTTTTATGCCTTGAATGTAGAAAATATTTTCGGAACAAGGTAGAGAAGATAATATTCTAATTAAATCGCAGAAAATATATTAAAAATTGAAAAAAAAAAACCGATTTTTCCCAAAAAATATATTAAAAAACCGAAAAAATTCCAATTTCTTCTTTAAAAAAATTTAAAAACATTGAAAAATGCTAATTTTTCTTATAAAAAACGAGAAAATTCCGATTTTTCTCTAAAAATATATTAAAAATTGAAATTATTCCGATTTTTCAGTAAAAATATCAAAAAATCAGATTTTTCTCTAAAAATACATTAAAAAATCAGATTTTTCTCTAAAAATACATTAAAAAATCCGTTTTTTTTTTAAATTTATATTAAAAATCGAAAAATTCCGATTTTTCTCTTTAAAAAATCGAAAAAATTGCAATTTTCTCAAAAAAAAATCGAAAAAAAAATTCCAATTTTTCTTTAAAAATATAAATATATATATAAAAAAGCGGAAAAATTCTGATTTTCTCAAAAAAAAAATTACAAATTAAAAAAATCCGACTTTTCTCTAAAAAAATTATTAAAAATCGAAAAAGATTCCTATTTTAATTTAATACAAAAATTAAAAATCGAAAAAAAAATTCGATTTTTCTTTTAAAAAATTTAAAAAATTGAAACATTTCCGATTTTTCTCTAAAAATATATTAAAAGAATCGAAAAAATTCCGATTTTTCTCACAAAATAATTTAAAAAATCAGAAATTTTTTTTTTTTAAACGAATATTTCAATTTTAATTCAAAAAATTTCATTTTTCAGCGCGAGTTCTATTGGCACACCGAAGATTGTCTTATGGAAGCTTTTGAACGAGAAGTTGCTCTAAGCGTATCCCGTGGAGCCGACGAAGATATCGCTGAAAATACTAGT

KI verification primers: Y56A3A.28-NKIF: 5'-GGAATTTTTTTGAAATTTTTAAGGAGA-3' Y56A3A.28-NKIR: 5'-TTCTTCTGATGGTTGATCATCCC-3'

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Strain name: SYS508

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; Y56A3A.28 (devKi140 [mNeonGreen::Y56A3A.28]) III

Expression pattern: Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): NHR-35, C16A3.4, CEH-5, PAG-3, BAZ-2, Y73E7A.1, UNC-39, Y48G1C.6, VAB-3, CEH-41, HLH-30, LIN-26, GMEB-1, NHR-25, TAB-1, HAM-2, NHR-23, UNC-130, LIN-1, LIN-32, CEH-32, ALR-1, SOX-2, REF-2, ATF-7, NFI-1, SPTF-1, CEH-43, CRH-2, CEBP-1, EGL-44, ELT-1, Y75B8A.6, HBL-1, EFL-3, ZIP-8


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NFYA-1

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: CEH-48, NFYA-1, DPL-1, EFL-1