WormBase ID: WBGene00003628
WormBase description: Is an ortholog of human HNF4A (hepatocyte nuclear factor 4 alpha) and HNF4G (hepatocyte nuclear factor 4 gamma). Is predicted to have DNA-binding transcription factor activity and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is expressed in intestine. Human ortholog(s) of this gene are implicated in maturity-onset diabetes of the young type 1 and type 2 diabetes mellitus.
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KI information: mNeonGreen knockin at C-terminus
sgRNA1: GACTACTATGAGTAAGTGTT
sgRNA2: TGGTTGTAGCTAGAAGTGAA
5'HR: GCATGCCATATGCATTTTTCAGCTCATATAAATTGTAGTCAAACAATACGCACAAAACCTTGAACACATTAGAATAAAACACACAATGTAAATGTTCCTGTTATTTTTTAAAGTGATGCACATTCTGTGGCTGCCCATAGAACTTGCGTAGTCATGAAAAAAATTACTACACTTTGAAATGCTAAACCTCGGTGCATTTTGTGGATAACTAGTTAAATAACAAGTTATATAACGATAACGACAACTGAAATATGAGCTGCCAAAGTTGAACAATGTGTTGCAATACTGATAGAGGAAATAAGGGCAAACATAAACCCAGGGTTTAACTGTTTAGTAAAAAGCCACCGGTTTTATTGATTAATTCAAAATCAAACAATTAAGCTCATTTTCGCAAAGCTGTTCCCAATTTTCAATGTTCCCTCTTTCTGTGTTTTGCAAATTATGAAAGCTCCGAAATTTCAAGAATCACATAGCATTTCCTTTTAATTACCCTATTGGGATATCTCACGCGTTCCCTTTTCTTGCAAAACCATAAATTCAACACACTTGAATATTAACTTTTTTGTGATCCCTTGCGGTTAATAATGCCACCGTGGTGAAGGCACTTGAAAAATTTATTCCACTTTTTCCACGTCTTCCGGGATTTGCAAAACCCCGTGAGTTGGCGGTGAATATTTTCAAGACCACCACTAAATCAAACTAGCAGAACGATCGAACATTAATGTGAAGAAAATTTTGCAGTTTCAAATTCAATTGTCACTTTTTTTGCAGATCTCTCACTGCCGGCCTCTTGCTCAAAACAGACGATGCTATGATGTCAGGAATCGGAACACAATACACAACTCCACAGTAAGAAGTTTTTATTATAAGTTTTAATTCTAAAAATGTTACATTTCAGACCTCACACACCACAATTTGCCGACTCCTCTCACCTAAACCTGCCGTATGCTCCGTTTACCTCCAGTTACAACCAATATCCAAACACTTACTCAGGTACC
3'HR: CTGCAGTAGTCGATTCTCAAAAAAAACTTTTTAATCCAAGTGTCTATTCTATCTATCCGTTTCATGTTCTCAAACTGTGATATTTGACAATGCCCGAAAATTCACCAGAAAAAAGTTATTTGAACTCCCAGAAAATGCCATTGAAACACAATTTGACGGGTTAGAGCAAACTCATTTGTTAAGAATATATTTTGTTTCCCACCCCCTAATAAATATAATTTTTATAATTTTCTCACAGTTTTCAATGATCTGTTATAAAACACAATCTTTTCATAAAATCTTTGAATCTGAAATCTGAATAGTTTTTCATCGTTCCACACACCCCAAAATCCAAACAGGATTTTAATTTACGACATCCCCTCTTCTCGGTGCTCGTCTTGTTCGAGGGCGTGAAGCGCGTTATTACATTTTGTTTGGCATTTGGCAGTGCACCCCTAAAAATGGACGGAGATGGCAAGAGGGAAGTGATGTTTCAGTGAAGTCATGCTGAAAATTGAGGTCCATTTTGATGCACACTCCAGCCAGGTGCGTGGGTTCTTTGAAAAAATTCGCAGATGCTTTCTAAATGGAGTTGAAACAAAGTTTGAGAATTTACAAGTTGTTCGAAAATATATTATTCACTGAGCCATTCGATCGAAATTTTGCCAATCCTCTCCCTAGTCTGTAAAAATTACCATCCGGAGAACAAAAATCAGTTCATCTCTTAGCCAGTCATGTGCACCATATTAATGAGAACCACGTCAGGGTATTGCTATTACAATTGGACTTTGATGGAAAATGTTCCGGACGTGACACATGAATATGTGGACTACTACTTTTTTTGCACGAATGTGTGTATGCAAATGTGATAGACTAATTTACATTATATACGTCAGTTATTATTAAGAACATGAAAGCAAAAATTTGAATAATTCATAGAAGATTAAACTTTTGACTAGT
KI verification primers: NHR-35-CKIF: 5'-TATTGTTCACGAATTTTGCTGGA-3' NHR-35-CKIR: 5'-CATCGCGGTATAACTGTGCTAGAT-3' Download
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Strain name: SYS528 KI background: JIM113 Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; nhr-35 (devKi156 [nhr-35::mNeonGreen]) X
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Expression pattern: Skin-specific
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): C16A3.4, NHR-102, CEH-40, NHR-23, Y56A3A.28, UNC-130, LIN-26, HAM-2, NHR-25
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: HAM-2, CEH-18, CEH-19, CEH-83, ELT-1, ELT-2, HLH-1, HLH-30, HMG-4, NFYA-1, RNT-1, TBX-2, UNC-120, UNC-55, UNC-62, C16A3.4, UNC-130, ALY-2, BLMP-1, DPL-1, EFL-1, ELT-3, FKH-8, FOS-1, IRX-1, LIN-13, LSY-2, MDL-1, MEP-1, NHR-85, SKN-1, SOX-4, SWSN-7 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: HAM-2, CEH-18, CEH-19, ELT-2, HLH-1, HLH-30, HMG-4, NFYA-1, RNT-1, TBX-2, UNC-120, UNC-55, UNC-62, C16A3.4, UNC-130, BLMP-1, DPL-1, EFL-1, ELT-3, FKH-8, IRX-1, LSY-2, MDL-1, MEP-1, NHR-85, SOX-4, SWSN-7
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