HBL-1

WormBase ID: WBGene00001824

WormBase description: Is an ortholog of several human genes including SCAND1 (SCAN domain containing 1); ZSCAN29 (zinc finger and SCAN domain containing 29); and ZSCAN5A (zinc finger and SCAN domain containing 5A). Exhibits RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including negative regulation of transcription by RNA polymerase II; nematode larval development; and oviposition. Localizes to nucleus. Is expressed in several structures, including body wall musculature; germ line; hypodermis; neurons; and ventral nerve cord. Human ortholog(s) of this gene are implicated in gingival fibromatosis 5.

KI information: mNeonGreen knockin at C-terminus

sgRNA1: AACGAGGACGTCCTCATTAT

sgRNA2: AGACGAGGGTAAGGGGCTAT

5'HR: GCATGCACCATATGCTGCTCAACAAGCATCTAGACGTTGGGTTGATGGGATTGAGGAACTCTGTAATGTCACCTCTGAAATGCTCTGCTTGTGATTTTGTTGCAAGTTCTGCAGACGAAAAAATGCGGCATAGTATGTCTCATATTTTGAACTCATCTAATGTTCCAACCTCAATCGCCTCCCTCTACAATAGTTTGAACCTTCCAAGTTTCTCTCATGTGGCACCAGATAATGACAATGCACTCGAATCCATGGATTGCGATGTGAAGATTGACGATGACAACATCACTGAAAGTCATTGCTATGAAGAAATGGACCAGGGTAGTGACTCGGCTGTTTCACCAACTGGCAGTTCACAAAGTAAGCGGTTTCTGACCTTTTTTCTGTAAACAACTTTTTTTTGCAGTTTCATCCGGAGACGAGGAGACGAAAAAGTGCAAATCGTTGTCGTTAGAGCAAATAAGCGCAAGGGCTAATGGAAACAACAGCCCGATGAGCAACGACAGTGCAATGGAGAAAGATGGCGAGGTAAGCGTAAATTAAAACTTTAAAAGATTATATTTTATATTATTTCAGAGCGCTGATGACGCACCACATTCACCATCGGATACAACATCAGTTCCATCTCCACCTCTTCATTCGTCTTCAATCGTCGCGCCAATCCCTATTACTCCACAGCCGAATGAGTTTTTGCAGTCCATTCTTGCACAAGCTTCCCTACTCGGCCCCCTACTTGCAAACCGACCTAGTGCATTTTACTGTGATCATTGCAAAATTCCATTTGATGTAAGTTTAGAATAGAAAGATTTGAATAATTGAAAAGCAAACTATTTCTAAATTGTTTCAGACCCAGCAAGTATTGGACAGTCATATGAGATTCCACACACCAGGGAACCCGTTCATGTGCAGTGACTGTCAGTATCAGGCTTTCAATGAGCTAAGCTTTGCTCTCCACATGTACCAAGCCAGACACCAAGGTACC

3'HR: CTGCAGTGAGGACGTCCTCGTTAAGGAAACACTTCGCATAGCCCCTTACCCTCGTCTAGTGACCCATTCTGAAATCGGCAGATAGACTTTCATGTAGCTGGTTTAAGTTTTCTCTTCATTTTCTTTAACTTATCAATGTTCGGGCGTTACCACTTTTCTAATCATGGCCAGTTTCTTGCACGTACACCTCAACCGTCCATTTCCATGAAATCTCATACTTGTTACTGTTTTCTTTTACCTCTGATGTCACATTTTCTCTCTGTCTCACTTTCTACCTCCAAACATATTTTACTACTTGTATTGAATGCCAAAAAATACCATATTTATTAAGGAGCATTGTTCATTTACAGTTTTGTACTCTCAGAGCGTGTTATTATCTAGAAGCAATTGTATACTGTTCTCAGTACATGTAGTACCTCCCCCAGAATACTTGTTTCTGTTACTATGTACCCCTCTTATTAACTTCGGGATATGAAACTTTTTATGTTTCATTTTCTATTGATTTCATTTGTTTGTCATTTTCAAGCTCCTCTTTCCACATAAGCTTTAACTGCATGTCTTTCATTTTTATTTATTTCTATTTGCCAATTGTTTAACTATGCACACATTTGTTTCATGTTTCTCCAGAGATAACTTTCCCAAATTCAAGTTTGCGCCAACTCGTGCTGCTCTTTTATTGTACGGTTTTATAACGTTTCCGTCTTGAAATCAGAGATTGTAGCCGTTTTTTGAAAAGGATATGCCAAAGAATCGTCCCCCACCCTCTAGTTGTCATTTGTTAAATAGCCGAAGTGACCCAACAACCCGCTTTTGTCCCCTCTACTAATAACCGTTTTATTATTATTATCACTCAATATTTATCTTTTTATGTACTTCTTTCACTGCTCCCATGTCGTGATTTCTGATTTCACATTTTCCAGACTATCTCGCACTTTCATTCTACCTCAATACATACTAGT

KI verification primers: HBL-1-CKIF: 5'-AAGAGATCAAAACCGAAACAGTTG-3' HBL-1-CKIR: 5'-AAAAAAGTAATCGGTGGATACAGGT-3'

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Strain name: SYS641

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; hbl-1 (devKi198 [hbl-1::mNeonGreen]) X

Expression pattern: Non-specific

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): LIN-22, ELT-3, SNAI-1, SYD-9, RNT-1, EGL-5, NHR-23, LIN-39, BLMP-1, NHR-12, GMEB-1, EGL-38, CEH-40, LIN-26, CEH-16, PQM-1, NOB-1, BAZ-2, HAM-2, CEH-20, NHR-102, NHR-232, DVE-1, COG-1, NHR-25, VAB-7, PHP-3, ATF-7, M03D4.4, REF-1, UNC-62, Y75B8A.6, HLH-1, VAB-15, CES-2, LIN-1, TLP-1, CEBP-1, F23B12.7, ELT-1, CRH-2, UNC-120, PAL-1, IRX-1, TBX-2, Y56A3A.28


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: SNAI-1, SYD-9, RNT-1, HAM-2, NHR-232, COG-1, M03D4.4, UNC-62, HLH-1, ELT-1, UNC-120, TBX-2, ALY-1, CEH-18, CEH-19, CEH-2, CEH-31, CEH-34, CEH-6, CEH-83, CND-1, DMD-4, ELT-2, EYG-1, FKH-6, HLH-15, HLH-8, HMG-4, LIN-13, LIN-32, MED-1, MLS-2, NFYA-1, PHA-4, PROS-1, REF-2, SOX-4, SPTF-1, UNC-42, UNC-55, UNC-86, ZTF-11, ELT-3, BLMP-1, IRX-1, CEH-8, CEH-82, DSC-1, FKH-10, LSY-2, MEP-1, NHR-85, UNC-130

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: SNAI-1, M03D4.4, ELT-1, CEH-18, CEH-31, CEH-6, CEH-83, DMD-4, HMG-4, LIN-32, MLS-2, PHA-4, SPTF-1, UNC-42, ELT-3, RNT-1, BLMP-1, IRX-1, TBX-2, CEH-8, CEH-82, DSC-1, FKH-10, LSY-2, MEP-1, NHR-85, UNC-130