HLH-3

WormBase ID: WBGene00001950

WormBase description: Is an ortholog of human ASCL1 (achaete-scute family bHLH transcription factor 1). Exhibits protein heterodimerization activity and sequence-specific DNA binding activity. Contributes to RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in several processes, including generation of neurons; regulation of oviposition; and transcription by RNA polymerase II. Localizes to RNA polymerase II transcription factor complex. Is expressed in several structures, including AB lineage cell; ABplapppapp; ABplpapaaa; nerve ring; and neurons.

Reporter verification: Verified by PCR and sequencing
Primers used to verify fosmid reporter: 5'-ACTCCAGAACAGTCTCCCGTAT-3'

Strain name: SYS222

Cross: OP650 x JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; wgIs650 (hlh-3::TY1::EGFP::3xFLAG + unc-119(+))

Expression pattern: Lineage-specific, Neuronal-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): LIM-4, DAF-19, CFI-1, UNC-42, TTX-3, CEH-48, TBX-37, UNC-30, EGL-46, NGN-1, NHR-67, CEH-39, FKH-2, TTX-1, HLH-2, LIN-32, ZAG-1, TBX-38, EGL-44, SPTF-1, CEH-36, ZTF-11, CEH-32


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: NA