TBX-37

WormBase ID: WBGene00006556

WormBase description: Is an ortholog of several human genes including EOMES (eomesodermin); TBX19 (T-box transcription factor 19); and TBX6 (T-box transcription factor 6). Is predicted to have DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II activating transcription factor binding activity; and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in pharynx development. Localizes to nucleus. Is expressed in head neurons. Human ortholog(s) of this gene are implicated in adrenocorticotropic hormone deficiency; atrial heart septal defect 4; and dysostosis (multiple).

KI information: mNeonGreen knockin at N-terminus

sgRNA1: CACCTCTCCTTCTGGAATTG

sgRNA2: TAGAACTTTTCCCAAATCTC

5'HR: GCATGCATTGGCTCACAGTTTGGTGAGTTTTCAATTTTTTTTTTGGTATTTTTTCGTCTTTTTTGCTAATTCGTGGTGTAGCTCATCATTATTACTATGGTGTGAATTATTTTGAATTTTTTTGTTAAAAATTTTTTGGCTAGAAGTTCAGTAAATTGACACTTATAGGGTGTAAAAATGTGAAAAAACCAGCTAATAACTTTATGCCAAAAATTCTGGAAAATAAAATATCATTAAAATTGAATTACTTTAAATTTTGTGCAGGCAGACTACACTAACCTTATTGAAAAACCAAACGTTTGCTAAGAATTCCGTATTTTTATTTCTCCAAAAAACACATTTGCCGAATTTGCTGAATTTGTCCAGCTCGGCAAATTTTAAGATTTGCCGCACACCTCTGGTTTCAACCAAAAAAAGGTTTAAAAAAATGCTATAAAATTCGAATTTCCGCGCATTTTTTGATTTTCTAGAAAGTTCTTTTTACGTCTTCGAGTATGAAATAAAATTTGAGCTTCTGTTTAAAGTTATCTGCTCGCGAATTTCAAATCCAACATTTCCAGTGCTAACGGGGGTTTTCCATCTTTAATATTTATAAATTTCAACTACACTTTTCCAGTGTTCCCCCGTTAAGAATACAGCCTAACCCTCTGTTCTTCAATTAGGAGAAGGGCCCCCATGCGTTCATATGTATGTACCAAGTTGTGCCCGACTGCCACGTGTCCACCACTACGCGTGATCTACCTCTTCCCGCTCCTCCCACTTACCGGCTCAGTTTCAGAGCATGAGTGAGAGTGTTGTTTGCACTTTTATTACAATTAGCGCCATTATTATTATTTTATTGTTTTACTTCCGTTCTCACCTTTTGAAATCTTTTGTATTAATATTTTCATAATAATTTCCCTCTATTACAGCATCACTGGTACC

3'HR: CTGCAGATGTACTCGTGCACCTCTCCTTCTGGAATTGAAGTCTCTTTGAACAAGCCGGAAATCTGGGAGAAATTCTACCCGAAAACTGAAATGATTGTGACGAGAAAGCGTGGGAGAGTCATTTTTCCGCATTTGGATTATAACGTCAAAGGATTGGATCCGGATAGTCTCTACTCGATTTATATTCATTTGGAACGTGTTGACGGGATTAAGTGAGTTGGAATAAATGATAGAAAAAAGTTGTCAACCAGTAAGTAAATTGTAGGAAATTAAATTTACTACAATTTGGTAGTTGACAACTTTTTGATTAGATGGATGCCTAGAGAGATATACAAGTTTAAAGTTGGAATTTTAAACTGCTATAGCATAGAGTAAATTTATTTTGCTTTAATTGACGTAAATATTGAACTGTGATGACCAAATATAGCCGTTAAAATTTTTTCTAAATTTTTTAAATGATTTTTCAAACTGATTTTTTCCGGATTAAGTTTGCCAGGATATTTCGAAAAAGTGAGTGGAGTCTAATCTCCCATTTTTTTTTGACAAAATTAGAAAAAAATCATAAATTATTTAGAAAAGTTGGAAAATTTTTTTAACAGTTATATTTGGTCATTACGGGCTATTTTCTGTCACTTGAACCAAAAATTGTTTCTTCTCAGCTTCTGAAGTTCCAGCGCCCGCAACTCTTTTAGGAATTGCCCTATAGAAAAGCAGTTAACTAACAGTTTGTAGATATTTGAATTTTCTACAGTTTTTTAGTTGACAAAATTTTGATAACTTTTGTAGTTTTTGAAATATTGATGCTAATAATGCCCCTCAATATTAAAAATCGGAAAAATCGGATAAAAAGCGGATTTTAATGGGGTTATTCAAGTAATTTAGCAAAATGTATTGAAATACATTTGTGACGTCACAAATGTATTTAAATAACTAGT

KI verification primers: TBX-37-NKIF: 5'-CAGGAGCACAAAGATGCTCAGTG-3' TBX-37-NKIR: 5'-GTCACAACTGTATTCAAGTATATAAAAACATG-3'

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Strain name: SYS400

KI background: JIM113

Strain genotype: ujIs113 (pie-1p::mCherry::H2B; nhr-2p::mCherry::his-24::let858 3'UTR; unc-119(+)) II; tbx-37 (devKi91 [mNeonGreen::tbx-37]) III

Expression pattern: Lineage-specific, Transient-expression

Lineage expression pattern

350 stage

600 stage

3D expression pattern

4-cells

8-cells

15-cells

28-cells

~50-cells

~100-cells

~200-cells

~350-cells

~500-cells

~600-cells

600-cells

bean-stage

Inferred TF relationships

Based on expression similarity (strong, medium, weak): TBX-38, CEH-39, T07F8.4, TBX-11, HLH-3, CEH-83, ETS-7, SEA-1


Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ZIP-8

Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)

Targeting: no-data

Targeted by: ZIP-8