WormBase ID: WBGene00006557
WormBase description: Is an ortholog of several human genes including EOMES (eomesodermin); TBX19 (T-box transcription factor 19); and TBX6 (T-box transcription factor 6). Is predicted to have DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II activating transcription factor binding activity; and RNA polymerase II regulatory region sequence-specific DNA binding activity. Is involved in pharynx development. Localizes to nucleus. Is expressed in head neurons. Human ortholog(s) of this gene are implicated in adrenocorticotropic hormone deficiency; atrial heart septal defect 4; and dysostosis (multiple).
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KI information: mNeonGreen knockin at N-terminus
sgRNA1: GACTTTAATTCCGGATGGGG
sgRNA2: CCAAATTTCTGGTGTGCTCA
5'HR: GCATGCCAATTTCAAAATGCACCAGCCCTCCCGCTGAGCCCGACACATCTTCTCCTTCCGAGCCAATTCAAAACTGCGCACCGCTATCACTAGAGTGTGAGGTGTCAAGGTGTGGGTGGAGGTATACTTTTGAAGTGTGAACTACACATTCTAGATTGCTGAAGACCATAGAGATGCTCAGTTGGAGGAAGAGGTGTTCAAGAATCAGACACCAACATTGGCTCGCAGTTTAATGAGTGTTCCGATTTTACAGTTATTTTGGATATACTTCGCGTGTGATCCTTCTAGGTCATTATTATATTGGTGTGAATTCAAAAAGCCTAAGCCTATGCTTTAGCCTAAACCTGAGCCTGAGCTGAGGTCTAAGCTTAAGCCTGAGCCTAAGCCTAAGCCTTAGCTTGAGCCTAAGCCTAATAAGGGATGGGCGGCGAACAGGCTTTCTTGGTGAACGGCGAATTCGGCGACTTTGCCTATGCAGTTTTTCCAGTTTTCCAGGTTTTCTCTGAAGGTTTTTTCTTGTTCAAAGGTGTAAGCCTAAGCCTAATTGTAAGCCTAAGCCCAAGCAGAAGCCTGACTGAGCCTAAACCTAAGCCTAGGCATAACTCTAAGCCTAAGCCTGAGCATAAGCCTATGTCTATGTCTAAGCCTAAACCTAAACCTAAAGACTAAGCCTAAGACTAAACCTAAAGACTAAGCCTAAGCCTGCACCCAAGGCCGCCGTGCGCTACATCTCCCATGGCCACACGCTCATATGTGCCAAGTTGTGCTCCACTTGCCACGTGTCCACCCCTACGTGTGGCCCAGCCCCTTCCGGCTCCGCCCACTTACCGGCTCAGTTTTACAGAATTAGTGAGAGTGGTTAGCACTTTTATTACAATTAGATTTTTGTTGCTCTACTTCCGGTCTCACTTTTTTGTGCTTCTGTTACTCCTATAACTCCCCCTGTTACAGCATTACCGTTACC
3'HR: CTGCAGATGTACTCGTGCACTTCCCCATCCGGAATTAAAGTCTCTTTGAGCACACCAGAAATTTGGGAAGAGTTCTACCCGAAAACCGAAATGATTGTGACGAGAAACCGTGGAAGGGTGATTTTTCCGCATTTGGACTATATCATCAAGGGATTGGATCCAGGCAGTCTCTACTCGATTTATATTCATTTGGAACGTGTTGACGGGATTAAGTGAGTTGGGTTAAAAGATAGAAAAAAATTTTCAACTGGTAAATTGTAGAAAATTAAATTTGCTACAATTTCTAAATTGACAACTTTTTGATAGCTGGTATACTTTCAGAGTTATAACCTATAGGACGATTCCAATTTCAAAAACTTGAACTCATTTCCAGATACAAATTCGACGCAGGAGAATGGAAAGAATTCGCCAAAGGAGATCCGATTCTTCCAATCCAATACAAGGAGCATCCGCGTGGCAAGCGTACTGGAGCAGAATGGATGAGTGAGCCCGTCTCGTTTGCTCACATCAAAATCACGAATAACCCGGAAATCAAGGATCAGAAGGTCATTTTAGTGCAGAGTATGCATAAGCACATTCCAGTGGTCACTGTTAAACAGGTCCGACATTATAAGACAGGGTATCAAGAGGATTTTAGTGGGGAGCAGTTCCGGTTGGAGGCTACTGAATTTATGGTGGTCACTGCTTATCAGGTTAGTTATTTGAAAGGGTGTGGGAAATTAAATTTGCAACAATTTGTTGAAAAAAGTTGAAAACATTTTAATCGCGTCACTATCAACGGAGATGAAGTAGTTTAAAGTTGGAGAGCATATTTTCAAGTTTCCAAAACTGTAAATTTCGAAGCGCTATTCCAACTTTTCAAATCAAAAATAAATGTAGACATATAGAAAGCATCTAATTCAAAATTAAAAAAATACTGAAAAACTTGCCGAAACTAGT
KI verification primers: TBX-38-NKIF: 5'-AGACATGTGCCGCTTATACTTTGC-3' TBX-38-NKIR: 5'-CTTAAGCTTCAGCAAATGGCAACT-3' Download
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Strain name: SYS422 KI background: RW10226 Strain genotype: itIs37 (pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)); stIs10226 (his-72p::his-24::mCherry::let-858 3' UTR + unc-119(+)); tbx-38 (devKi93 [mNeonGreen::tbx-38]) III
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Expression pattern: Lineage-specific, Transient-expression
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Lineage expression pattern
350 stage
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600 stage
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3D expression pattern  4-cells  8-cells  15-cells  28-cells  ~50-cells  ~100-cells  ~200-cells  ~350-cells  ~500-cells  ~600-cells  600-cells  bean-stage |
Inferred TF relationships
Based on expression similarity (strong, medium, weak): TBX-37, CEH-39, T07F8.4, TBX-11, TBX-8, CEH-41, TBX-9, SEM-2, LIM-7, CEH-83, ETS-7, HLH-3, CEH-43
Based on ChIP-seq (directly from ModERN database, targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: ETS-7, CEH-36 |
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 Based on reannotated ChIP-seq (peaks are assigned to a target if it is located within 2 kb relative to the transcription start site; targets from L1 stage ChIP-seq data are underlined)
Targeting: no-data
Targeted by: ETS-7, CEH-36
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